PMID- 22665231 OWN - NLM STAT- MEDLINE DCOM- 20121015 LR - 20120605 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 882 DP - 2012 TI - Simple methods for the detection of HLA-G variants in coding and non-coding regions. PG - 123-42 LID - 10.1007/978-1-61779-842-9_7 [doi] AB - The non-classical human leukocyte antigen (HLA)-G plays a crucial role in the induction of tolerance at the feto-maternal interface as well as in transplantation, cancer, inflammation, and autoimmune diseases. To understand gene regulation and the impact of polymorphic sites on the function, simple and easy feasible approaches are needed for the detection of HLA-G variants in coding and non-coding regions. Here we summarize a set of methods for the identification of variants in the exon 2-4, in the 3' untranslated region and in the gene promoter region of the HLA-G gene. FAU - Nuckel, Holger AU - Nuckel H AD - Department of Haematology and Institute of Pharmacogenetics, University Hospital Essen, Essen, Germany. FAU - Castelli, Erick C AU - Castelli EC FAU - Moreau, Philippe AU - Moreau P FAU - Ochsenfarth, Crista AU - Ochsenfarth C FAU - Horn, Peter A AU - Horn PA FAU - Rebmann, Vera AU - Rebmann V LA - eng PT - Journal Article PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 RN - 0 (3' Untranslated Regions) RN - 0 (HLA-G Antigens) SB - IM MH - 3' Untranslated Regions/*genetics MH - Exons/*genetics MH - Genetic Variation/*genetics MH - HLA-G Antigens/*genetics MH - Humans MH - Promoter Regions, Genetic/genetics MH - Sequence Analysis, DNA/*methods EDAT- 2012/06/06 06:00 MHDA- 2012/10/16 06:00 CRDT- 2012/06/06 06:00 PHST- 2012/06/06 06:00 [entrez] PHST- 2012/06/06 06:00 [pubmed] PHST- 2012/10/16 06:00 [medline] AID - 10.1007/978-1-61779-842-9_7 [doi] PST - ppublish SO - Methods Mol Biol. 2012;882:123-42. doi: 10.1007/978-1-61779-842-9_7.