PMID- 22666747
OWN - NLM
STAT- MEDLINE
DCOM- 20130220
LR  - 20191027
IS  - 1678-2674 (Electronic)
IS  - 0102-8650 (Linking)
VI  - 27
IP  - 5
DP  - 2012 May
TI  - Effective reinnervation of the quadriceps femoris by spinal ventral root 
      cross-anastomosis in rats.
PG  - 330-7
LID - S0102-86502012000500009 [pii]
AB  - PURPOSE: To study the effective recovery of the quadriceps femoris by spinal 
      ventral root cross-anastomosis in rats. METHODS: End-to-end anastomosis was 
      performed between the left L(1) and L(3) ventral roots using autogenous nerve 
      graft, and the right L(1) and L(3) roots were left intact. In control animals, 
      the left L(3) ventral root was cut and shortened, and anastomosis was not 
      performed. Six months postoperatively, the movement of low extremities was 
      detected by electrophysiological examination, hindlimb locomotion and basso, 
      beattie and bresnahan (BBB) scoring at one, three, seven, 14, 21 and 28 days 
      after SCI. Fluorescence retrograde tracing with TRUE BLUE (TB) and HE staining 
      were performed to observe the nerve regeneration. RESULTS: Six months after 
      surgery, the anastomotic nerve was smooth and not atrophic. The amplitudes of 
      action potential were 7.63 +/- 1.86 mV and 6.0 +/- 1.92 mV respectively before and 
      after the spinal cord hemisection. The contraction of left quadriceps femoris was 
      induced by a single stimulation of the anastomotic nerve. The locomotion of left 
      hindlimb was partially restored after spinal cord hemisection while creeping and 
      climbing. In addition, there was significant difference in the BBB score at one, 
      three and seven days after SCI. TB retrograde tracing and neurophysiologic 
      observation indicated efficient reinnervation of the quadriceps femoris. 
      CONCLUSION: The cross-anastomosis between spinal ventral root can partially 
      reconstruct the function of quadriceps femoris following SCI and may have 
      clinical implication for the treatment of human SCI.
FAU - Song, Chao
AU  - Song C
AD  - Department of Orthopaedic Surgery, School of Medicine, Shanghai Jiao Tong 
      University, Affiliated Renji Hospital, China.
FAU - Zhong, Gui-bin
AU  - Zhong GB
FAU - Liu, Zu-de
AU  - Liu ZD
FAU - Li, Wei
AU  - Li W
FAU - Ni, Peng-wen
AU  - Ni PW
FAU - Qiao, Zhi-guang
AU  - Qiao ZG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Acta Cir Bras
JT  - Acta cirurgica brasileira
JID - 9103983
SB  - IM
MH  - Anastomosis, Surgical
MH  - Animals
MH  - Femoral Nerve/*surgery
MH  - Male
MH  - Models, Animal
MH  - Nerve Regeneration/*physiology
MH  - Quadriceps Muscle/*innervation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function/physiology
MH  - Spinal Cord Injuries/*surgery
MH  - Spinal Nerve Roots/*surgery
EDAT- 2012/06/06 06:00
MHDA- 2013/02/21 06:00
CRDT- 2012/06/06 06:00
PHST- 2012/01/18 00:00 [received]
PHST- 2012/04/16 00:00 [accepted]
PHST- 2012/06/06 06:00 [entrez]
PHST- 2012/06/06 06:00 [pubmed]
PHST- 2013/02/21 06:00 [medline]
AID - S0102-86502012000500009 [pii]
AID - 10.1590/s0102-86502012000500009 [doi]
PST - ppublish
SO  - Acta Cir Bras. 2012 May;27(5):330-7. doi: 10.1590/s0102-86502012000500009.