PMID- 22667094
OWN - NLM
STAT- MEDLINE
DCOM- 20130712
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 1
DP  - 2012 Jan
TI  - [Preparation of immunoaffinity chromatographic column specific to diniconazole 
      and its applications to the pretreatment of samples].
PG  - 67-70
AB  - The purified anti-diniconazole antibody was polymerised to hydrolytic 
      tetramethoxysilane (TMOS) to synthesize the immunosorbent for the immunoaffinity 
      chromatographic (IAC) column specific to diniconazole. The optimized conditions 
      of the IAC were as follows: water as equilibrium and adsorbent medium, 30% and 
      50% (v/v) methanol aqueous solutions as eluents. The results showed that the 
      dynamic column capacity was up to 125.4 microg/g of bed volume. The river water 
      and fruit samples spiked with diniconazole were cleaned up and enriched by the 
      IAC, and the diniconazole in eluant was determined by high performance liquid 
      chromatography. The average recoveries (n = 5) of diniconazole in river water 
      sample were 90.36%-100.14% with relative standard deviations (RSDs) of 
      2.03%-6.08%, and the average recoveries in fruit samples were 85.55%-94.02% with 
      RSDs of 3.38%-6.78%. The IAC cleanup procedure provided an effective pretreatment 
      method for the determination of diniconazole in sample media such as river water 
      and fruits.
FAU - Jin, Yahui
AU  - Jin Y
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, 
      China.
FAU - Wang, Minghua
AU  - Wang M
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Pesticide Residues)
RN  - 0 (Triazoles)
RN  - 0 (Water Pollutants, Chemical)
RN  - X82HVO1N83 (diniconazole)
SB  - IM
MH  - Chromatography, Affinity/*instrumentation
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Food Contamination/analysis
MH  - Fruit/chemistry
MH  - Immunosorbent Techniques
MH  - Pesticide Residues/*analysis
MH  - Triazoles/*analysis/immunology
MH  - Water Pollutants, Chemical/analysis
EDAT- 2012/06/07 06:00
MHDA- 2013/07/16 06:00
CRDT- 2012/06/07 06:00
PHST- 2012/06/07 06:00 [entrez]
PHST- 2012/06/07 06:00 [pubmed]
PHST- 2013/07/16 06:00 [medline]
AID - 10.3724/sp.j.1123.2011.09036 [doi]
PST - ppublish
SO  - Se Pu. 2012 Jan;30(1):67-70. doi: 10.3724/sp.j.1123.2011.09036.