PMID- 22668575
OWN - NLM
STAT- MEDLINE
DCOM- 20130328
LR  - 20191210
IS  - 1533-0311 (Electronic)
IS  - 0193-1091 (Linking)
VI  - 34
IP  - 6
DP  - 2012 Aug
TI  - A proposal for improving multicolor FISH sensitivity in the diagnosis of 
      malignant melanoma using new combined criteria.
PG  - 580-5
LID - 10.1097/DAD.0b013e3182433f3a [doi]
AB  - Fluorescence in situ hybridization (FISH) for the diagnosis of melanoma makes use 
      of specific fluorescent probes to detect selected chromosomal alterations on 
      paraffin-embedded tissue samples. To date, interpretation of FISH data has been 
      based on numerical values generated by 2 different computational algorithms that 
      of Abbott and that of Gerami. To further evaluate the value of FISH in the 
      diagnosis of malignant melanoma, we selected 163 clinically and histologically 
      unequivocal cases of malignant melanoma in a cohort of 575 melanocytic tumors and 
      analyzed FISH data using the criteria of Abbott, Gerami, and new combined 
      criteria. Depending on the used criteria, FISH was positive in the unequivocal 
      malignant melanoma in 69.3% (113/163) of cases using the Abbott criteria, 74.2% 
      (121/163) of cases using the Gerami criteria, and 82.2% (134/163) of cases using 
      the combined criteria of Abbott and Gerami. Although use of all 3 criteria was 
      associated with 100% FISH negativity in a cohort of 30 unequivocal benign 
      melanocytic nevi, use of the combined criteria revealed more FISH-positive cases 
      in ambiguous benign melanocytic lesions than the criteria of Abbott or Gerami 
      alone: Abbott, 125 of 367; Gerami, 146 of 367; combined, 161 of 367. Furthermore, 
      we show that 66% (8/12) of FISH-negative cases of unequivocal melanoma are 
      positive when analyzed by array comparative genomic hybridization (aCGH), 
      demonstrating that false-negative results remain despite the usage of the 
      combined criteria for evaluation of FISH data. In these 8 FISH-negative 
      aCGH-positive cases, copy number alterations were often located on chromosomes 
      9p, a chromosomal locus that is not targeted by the FISH probes currently used. 
      In conclusion, the existing criteria for the evaluation of multicolor melanocytic 
      FISH are limited by a nonnegligeable rate of false negativity that can be reduced 
      by using newly proposed combined criteria but at the cost of increased detection 
      of FISH positivity in ambiguous benign melanocytic lesions.
FAU - Kerl, Katrin
AU  - Kerl K
AD  - Department of Dermatology, Zurich University Hospital, Zurich, Switzerland. 
      katrin.kerl@usz.ch
FAU - Palmedo, Gabriele
AU  - Palmedo G
FAU - Wiesner, Thomas
AU  - Wiesner T
FAU - Mentzel, Thomas
AU  - Mentzel T
FAU - Rutten, Arno
AU  - Rutten A
FAU - Scharer, Leo
AU  - Scharer L
FAU - Paredes, Bruno
AU  - Paredes B
FAU - Hantschke, Markus
AU  - Hantschke M
FAU - Kutzner, Heinz
AU  - Kutzner H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Am J Dermatopathol
JT  - The American Journal of dermatopathology
JID - 7911005
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - *Algorithms
MH  - Chromosomes, Human, Pair 9/genetics
MH  - Cohort Studies
MH  - Comparative Genomic Hybridization
MH  - DNA, Neoplasm/genetics
MH  - False Negative Reactions
MH  - Gene Dosage/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods/*trends
MH  - Melanoma/*diagnosis/genetics
MH  - Sensitivity and Specificity
MH  - Skin Neoplasms/*diagnosis/genetics
EDAT- 2012/06/07 06:00
MHDA- 2013/03/30 06:00
CRDT- 2012/06/07 06:00
PHST- 2012/06/07 06:00 [entrez]
PHST- 2012/06/07 06:00 [pubmed]
PHST- 2013/03/30 06:00 [medline]
AID - 10.1097/DAD.0b013e3182433f3a [doi]
PST - ppublish
SO  - Am J Dermatopathol. 2012 Aug;34(6):580-5. doi: 10.1097/DAD.0b013e3182433f3a.