PMID- 22668597
OWN - NLM
STAT- MEDLINE
DCOM- 20121024
LR  - 20211021
IS  - 1544-0591 (Electronic)
IS  - 0022-0345 (Print)
IS  - 0022-0345 (Linking)
VI  - 91
IP  - 8
DP  - 2012 Aug
TI  - Projection of non-peptidergic afferents to mouse tooth pulp.
PG  - 777-82
LID - 10.1177/0022034512450298 [doi]
AB  - A large proportion of pulpal nociceptors are known to contain neuropeptides such 
      as CGRP. However, the projection of non-peptidergic nociceptors to tooth pulp is 
      controversial. Recently, the non- peptidergic subset of nociceptors has been 
      implicated in mechanical pain in the skin. Since mechanical irritation of pulpal 
      nociceptors is critical for evoking tooth pain under pathophysiological 
      conditions, we investigated whether the non-peptidergic afferents project to 
      tooth pulp as potential mechanotransducing afferents. For clear visualization of 
      the non-peptidergic afferents, we took advantage of a recently generated knock-in 
      mouse model in which an axonal tracer, farnesylated green fluorescence protein 
      (GFP), is expressed from the locus of a sensory neuron-specific gene, Mrgprd. In 
      the trigeminal ganglia (TG), we demonstrated that GFP is exclusively expressed in 
      afferents binding to isolectin B4 (IB4), a neurochemical marker of 
      non-peptidergic nociceptors, but is rarely co-localized with CGRP. Retrograde 
      labeling of pulpal afferents demonstrated that a low proportion of pulpal 
      afferents was co-localized with GFP. Immunohistochemical detection of the axonal 
      tracer revealed that GFP-positive afferent terminals were densely projected into 
      the tooth pulp. These results provide convincing evidence that non-peptidergic 
      nociceptors are projected into the tooth pulp and suggest a potential role for 
      these afferents in tooth pain.
FAU - Chung, M-K
AU  - Chung MK
AD  - Department of Neural and Pain Sciences, School of Dentistry, University of 
      Maryland, Baltimore, MD 21201, USA. mchung@umaryland.edu
FAU - Jue, S S
AU  - Jue SS
FAU - Dong, X
AU  - Dong X
LA  - eng
GR  - DE019694/DE/NIDCR NIH HHS/United States
GR  - DE020866/DE/NIDCR NIH HHS/United States
GR  - R01 GM087369/GM/NIGMS NIH HHS/United States
GR  - GM087369/GM/NIGMS NIH HHS/United States
GR  - R03 DE020866/DE/NIDCR NIH HHS/United States
GR  - K08 DE019694/DE/NIDCR NIH HHS/United States
GR  - NS054791/NS/NINDS NIH HHS/United States
GR  - R01 NS054791/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120605
PL  - United States
TA  - J Dent Res
JT  - Journal of dental research
JID - 0354343
RN  - 0 (Lectins)
RN  - 0 (Luminescent Agents)
RN  - 0 (Mrgprd protein, mouse)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Afferent Pathways/anatomy & histology
MH  - Animals
MH  - Calcitonin Gene-Related Peptide/analysis
MH  - Dental Pulp/*innervation
MH  - Gene Knock-In Techniques
MH  - Green Fluorescent Proteins
MH  - Lectins/analysis
MH  - Luminescent Agents
MH  - Mechanoreceptors/classification
MH  - Mechanotransduction, Cellular/physiology
MH  - Mice
MH  - Mice, Transgenic
MH  - Nerve Endings/classification/ultrastructure
MH  - Nerve Fibers/classification/ultrastructure
MH  - Neurons, Afferent/classification
MH  - Nociceptors/*classification
MH  - Receptors, G-Protein-Coupled/analysis/genetics
MH  - Trigeminal Ganglion/cytology
PMC - PMC3398789
COIS- The authors declare no potential conflicts of interest with respect to the 
      authorship and/or publication of this article.
EDAT- 2012/06/07 06:00
MHDA- 2012/10/25 06:00
PMCR- 2013/08/01
CRDT- 2012/06/07 06:00
PHST- 2012/06/07 06:00 [entrez]
PHST- 2012/06/07 06:00 [pubmed]
PHST- 2012/10/25 06:00 [medline]
PHST- 2013/08/01 00:00 [pmc-release]
AID - 0022034512450298 [pii]
AID - 10.1177_0022034512450298 [pii]
AID - 10.1177/0022034512450298 [doi]
PST - ppublish
SO  - J Dent Res. 2012 Aug;91(8):777-82. doi: 10.1177/0022034512450298. Epub 2012 Jun 
      5.