PMID- 22669506
OWN - NLM
STAT- MEDLINE
DCOM- 20121119
LR  - 20220321
IS  - 1432-0584 (Electronic)
IS  - 0939-5555 (Linking)
VI  - 91
IP  - 10
DP  - 2012 Oct
TI  - Flow cytometric quantification and immunophenotyping of leukemic stem cells in 
      acute myeloid leukemia.
PG  - 1541-6
LID - 10.1007/s00277-012-1501-7 [doi]
AB  - Leukemic stem cells (LSCs) are root of clonal growth in acute myeloid leukemia 
      (AML) and responsible for the propagation of leukemic blasts (LBs). LSCs are 
      considered as CD34 + CD38- population among LBs and often express as CD123, CD44, 
      or CD184, which are rarely expressed on normal hematopoietic stem cells and could 
      be the potential therapeutic targets. Using multi-color flow cytometry, we 
      analyzed the proportions of CD34 + CD38- LSCs and expression of CD123, CD44, and 
      CD184 on LSCs in 63 patients with AML. The median proportion of LSCs was 1.3 % 
      (0.0-33.1 %) at the time of diagnosis. Of all patients, 74.6 % of them had 
      CD123-positive LSCs, all patients had CD44-positive LSCs, and 85.7 % had 
      CD184-positive LSCs, respectively. The proportions of LSCs were significantly 
      lower in the complete remission (CR) group compared with non-CR group (P = 
      0.006). The lower proportions of LSCs in CR group indicated that measurement of 
      the proportion of LSCs might be helpful to predict the prognosis of AML.
FAU - Hwang, Keumrock
AU  - Hwang K
AD  - Department of Laboratory Medicine, University of Ulsan College of Medicine and 
      Asan Medical Center, 388-1 Pungnap-2dong, Songpa-gu, Seoul 138-736, South Korea.
FAU - Park, Chan-Jeoung
AU  - Park CJ
FAU - Jang, Seongsoo
AU  - Jang S
FAU - Chi, Hyun-Sook
AU  - Chi HS
FAU - Kim, Dae-Young
AU  - Kim DY
FAU - Lee, Jung-Hee
AU  - Lee JH
FAU - Lee, Je Hwan
AU  - Lee JH
FAU - Lee, Kyoo Hyung
AU  - Lee KH
FAU - Im, Ho-Joon
AU  - Im HJ
FAU - Seo, Jong-Jin
AU  - Seo JJ
LA  - eng
PT  - Journal Article
DEP - 20120606
PL  - Germany
TA  - Ann Hematol
JT  - Annals of hematology
JID - 9107334
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Flow Cytometry/*methods
MH  - Follow-Up Studies
MH  - Humans
MH  - Immunophenotyping/*methods
MH  - Infant
MH  - Leukemia, Myeloid, Acute/diagnosis/*genetics/*immunology
MH  - Male
MH  - Middle Aged
MH  - Neoplastic Stem Cells/*immunology
MH  - Young Adult
EDAT- 2012/06/07 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/06/07 06:00
PHST- 2012/02/15 00:00 [received]
PHST- 2012/05/22 00:00 [accepted]
PHST- 2012/06/07 06:00 [entrez]
PHST- 2012/06/07 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 10.1007/s00277-012-1501-7 [doi]
PST - ppublish
SO  - Ann Hematol. 2012 Oct;91(10):1541-6. doi: 10.1007/s00277-012-1501-7. Epub 2012 
      Jun 6.