PMID- 22672085
OWN - NLM
STAT- MEDLINE
DCOM- 20130109
LR  - 20131121
IS  - 1366-5804 (Electronic)
IS  - 1354-750X (Linking)
VI  - 17
IP  - 6
DP  - 2012 Sep
TI  - Robust changes in expression of brain-derived neurotrophic factor (BDNF) mRNA and 
      protein across the brain do not translate to detectable changes in BDNF levels in 
      CSF or plasma.
PG  - 524-31
LID - 10.3109/1354750X.2012.694476 [doi]
AB  - Adult rats were treated acutely with peripheral kainic acid (KA), and changes in 
      brain-derived neurotrophic factor (BDNF) mRNA and protein were tracked over time 
      across multiple brain regions. Despite robust elevation in both mRNA and protein 
      in multiple brain regions, plasma BDNF was unchanged and cerebrospinal fluid 
      (CSF) BDNF levels remained undetectable. Primary neurons were then treated with 
      KA. BDNF was similarly elevated within neurons, but was undetectable in neuronal 
      media. Thus, while deficits in BDNF signaling have been implicated in a number of 
      diseases, these data suggest that extracellular concentrations of BDNF may not be 
      a facile biomarker for changes in neurons.
FAU - Lanz, Thomas A
AU  - Lanz TA
AD  - Pfizer Global Research and Development, Groton, CT 06340, USA. 
      thomas.a.lanz@pfizer.com
FAU - Bove, Susan E
AU  - Bove SE
FAU - Pilsmaker, Catherine D
AU  - Pilsmaker CD
FAU - Mariga, Abigail
AU  - Mariga A
FAU - Drummond, Elena M
AU  - Drummond EM
FAU - Cadelina, Gregory W
AU  - Cadelina GW
FAU - Adamowicz, Wendy O
AU  - Adamowicz WO
FAU - Swetter, Brentt J
AU  - Swetter BJ
FAU - Carmel, Sharon
AU  - Carmel S
FAU - Dumin, Jo Ann
AU  - Dumin JA
FAU - Kleiman, Robin J
AU  - Kleiman RJ
LA  - eng
PT  - Journal Article
DEP - 20120607
PL  - England
TA  - Biomarkers
JT  - Biomarkers : biochemical indicators of exposure, response, and susceptibility to 
      chemicals
JID - 9606000
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Animals
MH  - Brain/metabolism/pathology
MH  - Brain-Derived Neurotrophic Factor/*blood/cerebrospinal fluid/*genetics/metabolism
MH  - Cells, Cultured
MH  - Choroid Plexus/pathology
MH  - *Gene Expression
MH  - Hippocampus/*metabolism/pathology
MH  - Humans
MH  - Kainic Acid
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Male
MH  - Neurons/enzymology/metabolism
MH  - Primary Cell Culture
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seizures/blood/cerebrospinal fluid/chemically induced
EDAT- 2012/06/08 06:00
MHDA- 2013/01/10 06:00
CRDT- 2012/06/08 06:00
PHST- 2012/06/08 06:00 [entrez]
PHST- 2012/06/08 06:00 [pubmed]
PHST- 2013/01/10 06:00 [medline]
AID - 10.3109/1354750X.2012.694476 [doi]
PST - ppublish
SO  - Biomarkers. 2012 Sep;17(6):524-31. doi: 10.3109/1354750X.2012.694476. Epub 2012 
      Jun 7.