PMID- 22672247
OWN - NLM
STAT- MEDLINE
DCOM- 20130823
LR  - 20140731
IS  - 1600-0714 (Electronic)
IS  - 0904-2512 (Linking)
VI  - 42
IP  - 2
DP  - 2013 Feb
TI  - Expression of Wilms' tumor 1 (WT1) in oral squamous cell carcinoma.
PG  - 133-9
LID - 10.1111/j.1600-0714.2012.01182.x [doi]
AB  - BACKGROUND: The product of the Wilms' tumor gene, WT1 protein, is a tumor antigen 
      for various kinds of cancer, and WT1 peptide-based cancer immunotherapy is widely 
      anticipated as a new possibility for cancer treatment. The aim of this study was 
      to investigate the expression of WT1 from quantitative and morphological 
      perspectives in oral squamous cell carcinoma (OSCC), the most widespread 
      malignant neoplasm of the oral cavity. METHODS: Six OSCC cell lines and tissue 
      sections from 29 OSCC patients were analyzed. To detect WT1 expression, reverse 
      transcription-polymerase chain reaction analysis (RT-PCR), real-time PCR, Western 
      blots, and immunofluorescence flow cytometry for WT1 were performed on the cell 
      lines, and immunohistochemistry and fluorescence in situ hybridization (FISH) 
      were performed on the tissue sections. RESULTS: WT1 mRNA was found overexpressed 
      in one of the six OSCC cell lines, with expression levels higher than that seen 
      in human leukemia cell line (K562). Immunohistochemical analysis of tissue 
      sections showed overexpression of WT1 protein in two patients, concentrated 
      mainly in the cytoplasm of the outer one to three cell layers of the cancer 
      nests. This was consistent with the expression of WT1 mRNA observed by FISH. 
      Meanwhile, WT1 was not detected on normal oral epithelium. WT1 protein was 
      detected on actively proliferating cancer nests and even on elongated epithelial 
      ridge where new droplet-cancer-nests were being formed and starting infiltration 
      toward subepithelial layer. CONCLUSIONS: The results suggest that WT1 plays an 
      important role in the pathogenesis of some types of OSCC, particularly in 
      proliferation of the cancer cells.
CI  - (c) 2012 John Wiley & Sons A/S.
FAU - Mikami, Toshinari
AU  - Mikami T
AD  - Division of Anatomical and Cellular Pathology, Department of Pathology, Iwate 
      Medical University, Iwate, Japan. toshi_m@sea.plala.or.jp
FAU - Hada, Tomohiro
AU  - Hada T
FAU - Chosa, Naoyuki
AU  - Chosa N
FAU - Ishisaki, Akira
AU  - Ishisaki A
FAU - Mizuki, Harumi
AU  - Mizuki H
FAU - Takeda, Yasunori
AU  - Takeda Y
LA  - eng
PT  - Journal Article
DEP - 20120605
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
RN  - 0 (WT1 Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/*pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cell Separation
MH  - Cytoplasm/pathology
MH  - Epithelium/pathology
MH  - Female
MH  - Flow Cytometry
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Mouth Mucosa/pathology
MH  - Mouth Neoplasms/*pathology
MH  - Neoplasm Invasiveness
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - WT1 Proteins/*analysis
EDAT- 2012/06/08 06:00
MHDA- 2013/08/24 06:00
CRDT- 2012/06/08 06:00
PHST- 2012/06/08 06:00 [entrez]
PHST- 2012/06/08 06:00 [pubmed]
PHST- 2013/08/24 06:00 [medline]
AID - 10.1111/j.1600-0714.2012.01182.x [doi]
PST - ppublish
SO  - J Oral Pathol Med. 2013 Feb;42(2):133-9. doi: 10.1111/j.1600-0714.2012.01182.x. 
      Epub 2012 Jun 5.