PMID- 22672327
OWN - NLM
STAT- MEDLINE
DCOM- 20130212
LR  - 20140731
IS  - 1751-553X (Electronic)
IS  - 1751-5521 (Linking)
VI  - 34
IP  - 5
DP  - 2012 Oct
TI  - Application of an immune-magnetic cell sorting method for CD138-positive plasma 
      cells in FISH analysis of multiple myeloma.
PG  - 541-6
LID - 10.1111/j.1751-553X.2012.01433.x [doi]
AB  - INTRODUCTION: Interphase fluorescence in situ hybridization (FISH) analysis of 
      multiple myeloma (MM) may indiscriminately count signals of nonplasma cells, thus 
      decreasing specificity and sensitivity. We aimed to evaluate the usefulness of an 
      immune-magnetic sorting method for plasma cells in FISH analysis of MM and define 
      optimal sample preparation conditions. METHODS: Plasma cells were purified using 
      EasySep((R)) CD138 Positive Selection Cocktail and Magnetic Nanoparticles 
      (Invitrogen). We compared FISH results with and without plasma cell purification 
      for three sample preparation methods: direct harvest, 24-h culture, and 96-h 
      culture with interleukin-4 in five newly diagnosed MM patients. Archived fixed 
      bone marrow cells of 17 MM patients were also studied. RESULTS: The percentage of 
      abnormal cells identified was significantly higher with plasma cell purification 
      than without purification (median, 88.0%; range, 84.0-100.0%vs. 15.0%, 
      12.5-29.5%, respectively). The three sample preparation methods showed comparable 
      results. Immune-magnetic sorting also significantly increased the percentage of 
      abnormal cells identified in FISH analysis of archived fixed bone marrow cells (P 
      < 0.001). CONCLUSIONS: Immune-magnetic CD138-positive cell sorting significantly 
      increased the percentage of abnormal cells identified in FISH analysis of MM 
      samples for all sample preparation methods. This method could also be applied for 
      retrospective FISH analysis of archived fixed bone marrow cells.
CI  - (c) 2012 Blackwell Publishing Ltd.
FAU - Shin, S Y
AU  - Shin SY
AD  - Department of Laboratory Medicine, University of Ulsan College of Medicine and 
      Asan Medical Center, Seoul, Korea.
FAU - Jang, S
AU  - Jang S
FAU - Park, C-J
AU  - Park CJ
FAU - Chi, H-S
AU  - Chi HS
FAU - Lee, J-H
AU  - Lee JH
FAU - Lee, J H
AU  - Lee JH
FAU - Lee, K H
AU  - Lee KH
FAU - Suh, C
AU  - Suh C
FAU - Lim, S E
AU  - Lim SE
FAU - Seo, E-J
AU  - Seo EJ
LA  - eng
PT  - Journal Article
DEP - 20120601
PL  - England
TA  - Int J Lab Hematol
JT  - International journal of laboratory hematology
JID - 101300213
RN  - 0 (Syndecan-1)
SB  - IM
MH  - Cell Count
MH  - Cell Culture Techniques
MH  - Cells, Cultured
MH  - Flow Cytometry
MH  - Humans
MH  - Immunomagnetic Separation/*methods
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Multiple Myeloma/genetics/*metabolism/pathology
MH  - Plasma Cells/*metabolism
MH  - Reproducibility of Results
MH  - Syndecan-1/*analysis
EDAT- 2012/06/08 06:00
MHDA- 2013/02/13 06:00
CRDT- 2012/06/08 06:00
PHST- 2012/06/08 06:00 [entrez]
PHST- 2012/06/08 06:00 [pubmed]
PHST- 2013/02/13 06:00 [medline]
AID - 10.1111/j.1751-553X.2012.01433.x [doi]
PST - ppublish
SO  - Int J Lab Hematol. 2012 Oct;34(5):541-6. doi: 10.1111/j.1751-553X.2012.01433.x. 
      Epub 2012 Jun 1.