PMID- 22673847
OWN - NLM
STAT- MEDLINE
DCOM- 20130307
LR  - 20211021
IS  - 1536-4798 (Electronic)
IS  - 0277-3740 (Print)
IS  - 0277-3740 (Linking)
VI  - 31
IP  - 12
DP  - 2012 Dec
TI  - Neurotrophins and nerve regeneration-associated genes are expressed in the cornea 
      after lamellar flap surgery.
PG  - 1460-7
LID - 10.1097/ICO.0b013e318247b60e [doi]
AB  - PURPOSE: To determine the in vivo expression of neurotrophins (NTs) and nerve 
      regeneration-associated genes (RAGs) after surgically creating a hinged lamellar 
      corneal flap in thy1-YFP mice. METHODS: Lamellar corneal flaps with multiple 
      hinges were created in thy1-YFP mice. Mice were killed at weeks 2, 4, and 8. 
      Quantitative polymerase chain reaction was performed to determine the expression 
      of NTs and RAGs in the corneas after lamellar transection. Nerve growth factor 
      (Ngf), brain-derived neurotrophic factor (Bdnf), glial cell-derived neurotrophic 
      factor (Gdnf), neurotrophin 3, neurotrophin 5, small proline-rich repeat protein 
      1A (Sprr1a), growth-associated protein 43 (Gap43), and beta III tubulin (Tubb3) 
      gene expressions were analyzed. Whole-mount confocal immunofluorescence and 
      Western analyses were performed for localization and abundance of robustly 
      expressed genes. RESULTS: Sprouts of fine YFP-positive fronds emanating from 
      transected (injured) nerve bundles were seen in the flap area at 2 weeks onward. 
      Bdnf and Sprr1a were robustly and significantly expressed at 2 weeks 
      postoperatively (>2-fold increase in expression; P<0.05). Bdnf localized to 
      thy1-YFP+ cells in operated corneas. Sprr1a localized to corneal epithelial cell 
      membranes. At 8 weeks, none of the NTs and RAGs had increased expression. Bdnf 
      (rho=0.73, P=0.001) and Sprr1a (rho=0.76, P=0.001) showed a significant positive 
      correlation with beta III tubulin. CONCLUSIONS: The neurotrophin Bdnf and RAG 
      Sprr1a are robustly and significantly expressed during corneal nerve regeneration 
      in vivo.
FAU - Chaudhary, Shweta
AU  - Chaudhary S
AD  - Corneal Neurobiology Laboratory, Department of Ophthalmology and Visual Sciences, 
      College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
FAU - Namavari, Abed
AU  - Namavari A
FAU - Yco, Lisette
AU  - Yco L
FAU - Chang, Jin-Hong
AU  - Chang JH
FAU - Sonawane, Snehal
AU  - Sonawane S
FAU - Khanolkar, Vishakha
AU  - Khanolkar V
FAU - Sarkar, Joy
AU  - Sarkar J
FAU - Jain, Sandeep
AU  - Jain S
LA  - eng
GR  - P30 EY001792/EY/NEI NIH HHS/United States
GR  - EY001792/EY/NEI NIH HHS/United States
GR  - K08 EY018874-04/EY/NEI NIH HHS/United States
GR  - K08 EY018874/EY/NEI NIH HHS/United States
GR  - EY018874/EY/NEI NIH HHS/United States
GR  - P30 EY001792-34/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cornea
JT  - Cornea
JID - 8216186
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cornea/*innervation/surgery
MH  - Gene Expression Regulation/*physiology
MH  - Mice
MH  - Microscopy, Confocal
MH  - Nerve Growth Factors/*genetics
MH  - Nerve Regeneration/*genetics
MH  - Polymerase Chain Reaction
MH  - *Surgical Flaps
MH  - Trigeminal Ganglion/*physiology
PMC - PMC3612527
MID - NIHMS351708
COIS- Conflict of interest : None.
EDAT- 2012/06/08 06:00
MHDA- 2013/03/08 06:00
PMCR- 2013/12/01
CRDT- 2012/06/08 06:00
PHST- 2012/06/08 06:00 [entrez]
PHST- 2012/06/08 06:00 [pubmed]
PHST- 2013/03/08 06:00 [medline]
PHST- 2013/12/01 00:00 [pmc-release]
AID - 10.1097/ICO.0b013e318247b60e [doi]
PST - ppublish
SO  - Cornea. 2012 Dec;31(12):1460-7. doi: 10.1097/ICO.0b013e318247b60e.