PMID- 22674132
OWN - NLM
STAT- MEDLINE
DCOM- 20121004
LR  - 20220310
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 878
DP  - 2012
TI  - Fluorescence in situ hybridization for cancer-related studies.
PG  - 149-74
LID - 10.1007/978-1-61779-854-2_10 [doi]
AB  - Cytogenetic analysis of tumour material has been greatly enhanced over the past 
      30 years by the application of a range of techniques based around fluorescence in 
      situ hybridization (FISH). Fluorescence detection for in situ hybridization has 
      the advantage of including the use of a multitude of fluorochromes to allow 
      simultaneous specific detection of multiple probes by virtue of their 
      differential labelling and emission spectra. FISH can be used to detect 
      structural (translocation/inversion) and numerical (deletion/gain) genetic 
      aberrations. This chapter will deal with FISH methods to detect and localize one 
      or more complementary nucleic acid sequences (probes) within a range of different 
      cellular targets including metaphase chromosomes, nuclei from cell suspension, 
      and formalin-fixed paraffin-embedded FFPE tissue sections. Methods for the 
      efficient localization of probes to FFPE tissue cores in tissue microarrays 
      (TMAs) are also described.
FAU - Kearney, Lyndal
AU  - Kearney L
AD  - Haemato-Oncology Research Unit, Division of Molecular Pathology, The Institute of 
      Cancer Research, Surrey, UK.
FAU - Shipley, Janet
AU  - Shipley J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA Probes)
RN  - 0 (Genetic Markers)
RN  - 6SO6U10H04 (Biotin)
RN  - NQ1SX9LNAU (Digoxigenin)
SB  - IM
MH  - Biotin/metabolism
MH  - Cell Nucleus/genetics/metabolism
MH  - Chromosomes, Human/genetics
MH  - DNA Probes/metabolism
MH  - Digoxigenin/metabolism
MH  - Genetic Markers/genetics
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/genetics
MH  - Metaphase/genetics
MH  - Neoplasms/*genetics/*pathology
MH  - Paraffin Embedding
MH  - Tissue Array Analysis
MH  - Tissue Fixation
EDAT- 2012/06/08 06:00
MHDA- 2012/10/05 06:00
CRDT- 2012/06/08 06:00
PHST- 2012/06/08 06:00 [entrez]
PHST- 2012/06/08 06:00 [pubmed]
PHST- 2012/10/05 06:00 [medline]
AID - 10.1007/978-1-61779-854-2_10 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2012;878:149-74. doi: 10.1007/978-1-61779-854-2_10.