PMID- 22678158
OWN - NLM
STAT- MEDLINE
DCOM- 20121123
LR  - 20220317
IS  - 1573-7217 (Electronic)
IS  - 0167-6806 (Linking)
VI  - 134
IP  - 2
DP  - 2012 Jul
TI  - HER-2 gene amplification by fluorescence in situ hybridization (FISH) compared 
      with immunohistochemistry (IHC) in breast cancer: a study of 528 equivocal cases.
PG  - 743-9
LID - 10.1007/s10549-012-2101-x [doi]
AB  - The concordance rate between fluorescence in situ hybridization (FISH) and 
      immunohistochemistry (IHC) on evaluating HER-2/neu status is still controversial, 
      especially for the IHC 2+/equivocal cases. In this study, we investigated the 
      amplification of HER-2/neu gene by FISH in IHC (2+)/equivocal specimens to 
      clarify the correlation between HER-2/neu and clinicopathologic features of 
      breast cancers. HER-2/neu amplification was determined by FISH on 528 specimens 
      of breast carcinomas with equivocal expression of HER-2/neu protein by IHC 
      detection. 65.5 % of IHC 2+ patients were negative for HER-2/neu amplification, 
      29.0 % were positive and the remaining was equivocal. A statistically significant 
      inverse association was found between hormone receptor expression and HER-2/neu 
      amplification (P < 0.05). Furthermore, polysomy of CEP17 was detected in 60 % of 
      breast carcinomas. The results highlight the necessity of FISH test for further 
      categorization when breast carcinoma cases are scored 2+ by IHC.
FAU - Zhang, Han
AU  - Zhang H
AD  - Department of Pathology, the First Affiliated Hospital of Zhejiang University, 
      Qingchun Road 79#, Hangzhou, Zhejiang, China. z8354335@yahoo.com.cn
FAU - Ren, Guoping
AU  - Ren G
FAU - Wang, Xiaoling
AU  - Wang X
FAU - Zhao, Jing
AU  - Zhao J
FAU - Yao, Hongtian
AU  - Yao H
FAU - Bai, Yanfeng
AU  - Bai Y
FAU - Bo, Wang
AU  - Bo W
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20120608
PL  - Netherlands
TA  - Breast Cancer Res Treat
JT  - Breast cancer research and treatment
JID - 8111104
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Breast Neoplasms/diagnosis/genetics/*metabolism
MH  - Carcinoma/diagnosis/genetics/*metabolism
MH  - Chromosomes, Human, Pair 17
MH  - Female
MH  - *Gene Amplification
MH  - Gene Dosage
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Receptor, ErbB-2/*genetics
EDAT- 2012/06/09 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/06/09 06:00
PHST- 2012/04/07 00:00 [received]
PHST- 2012/05/16 00:00 [accepted]
PHST- 2012/06/09 06:00 [entrez]
PHST- 2012/06/09 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 10.1007/s10549-012-2101-x [doi]
PST - ppublish
SO  - Breast Cancer Res Treat. 2012 Jul;134(2):743-9. doi: 10.1007/s10549-012-2101-x. 
      Epub 2012 Jun 8.