PMID- 22678804
OWN - NLM
STAT- MEDLINE
DCOM- 20120911
LR  - 20120608
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 29
IP  - 3
DP  - 2012 Jun
TI  - [Clinical and experimental study of a multiple myeloma case with low 
      hypodiploidy].
PG  - 343-6
LID - 10.3760/cma.j.issn.1003-9406.2012.03.021 [doi]
AB  - OBJECTIVE: To report the clinical and laboratory characterization of a case of 
      multiple myeloma with low hypodiploid complex karyotyptic abnormalities. METHODS: 
      Cytogenetic examination of bone marrow performed by 24 h culture method. 
      R-banding technique was used to analyze the karyotype. Interphase fluorescence in 
      situ hybridization (FISH) was performed using chromosome probes such as 13q14, 
      p53, Rb1, 1q21 and IgH/CCND1. The DNA content was detected by flow cytometry. 
      RESULTS: Chromosome analysis revealed complex chromosomal rearrangement. Five 
      cells had a low hypodiploid karyotype with 35 chromosomes. Three cells had the 
      duplication of the low hypodiploid karyotype. Four cells had a normal karyotype. 
      Monosomy 1, 13, 14, 17 and a mark chromosome 1 derived from chromosome 11 
      resulting in the amplication of CCND1 gene were confirmed by interphase FISH. 
      Flow cytometric analysis displayed a low hypodiploid peak with the DNA index of 
      0.8426. CONCLUSION: These results indicated that the low hypodiploidy is a rare 
      abnormality in multiple myeloma. Interphase FISH is a reliable method for 
      detecting molecular abnormalities in multiple myeloma.
FAU - Bai, Shu-xiao
AU  - Bai SX
AD  - The First Affiliated Hospital of Soochow University, Jiangsu Institute of 
      Hematology, Thrombosis and Hemostasis, Key Laboratory of the Ministry of Health, 
      Suzhou, Jiangsu 215006, P. R. China.
FAU - Pan, Jin-lan
AU  - Pan JL
FAU - Xue, Yong-quan
AU  - Xue YQ
FAU - Chen, Su-ning
AU  - Chen SN
FAU - Wu, Ya-fang
AU  - Wu YF
FAU - Wang, Yong
AU  - Wang Y
FAU - Zhang, Jun
AU  - Zhang J
FAU - Shen, Juan
AU  - Shen J
LA  - chi
PT  - Case Reports
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - *Abnormal Karyotype
MH  - Adult
MH  - Cytogenetics/methods
MH  - Female
MH  - Gene Rearrangement/genetics
MH  - Humans
MH  - Multiple Myeloma/diagnosis/*genetics
EDAT- 2012/06/09 06:00
MHDA- 2012/09/12 06:00
CRDT- 2012/06/09 06:00
PHST- 2012/06/09 06:00 [entrez]
PHST- 2012/06/09 06:00 [pubmed]
PHST- 2012/09/12 06:00 [medline]
AID - 940629081 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2012.03.021 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2012 Jun;29(3):343-6. doi: 
      10.3760/cma.j.issn.1003-9406.2012.03.021.