PMID- 22679829
OWN - NLM
STAT- MEDLINE
DCOM- 20130712
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 2
DP  - 2012 Feb
TI  - [Determination of mitomycin C in rabbit plasma by ultra-high performance liquid 
      chromatography-tandem mass spectrometry].
PG  - 154-9
AB  - An ultra-high performance liquid chromatography-tandem mass spectrometry 
      (UHPLC-MS/MS) method for the determination of mitomycin C (MMC) in rabbit plasma 
      was established. The blank rabbit plasma sample solutions added with mitomycin C 
      and triamcinolone acetonide (internal standard, IS) were prepared. The solutions 
      containing MMC and IS were extracted from the plasma with ethyl acetate using 
      liquid-liquid extraction method. A Hypersil Gold C18 column (50 mm x 2.1 mm, 1.9 
      microm) was employed and the column temperature was set at 35 degrees C. The 
      isocratic elution of methanol and 0.1% (v/v) formic acid aqueous solution as 
      mobile phase was performed at a flow rate of 0.2 mL/min, and a rapid separation 
      was completed within 3 min. The electrospray was operated in the positive 
      ionization mode and the MMC and IS were identified in selected reaction 
      monitoring (SRM) mode. The monitoring ions of MMC and IS were m/z 335. 2 --> 
      242.2 and m/z 435.2 --> 397. 3/415.2, respectively, which were used to qualify 
      and quantify the targets by the method of matrix-matched standard solution. The 
      calibration curve showed good linearity within the mass concentrations of 1 to 1 
      000 microg/L (r = 0.997 8, weighting: 1/x2). The limit of detection (S/N = 3) was 
      0.2 microg/L. The recoveries were from 85% to 115% at the spiked levels of 1, 5, 
      100 and 800 microg/L, and the relative standard deviations (RSDs) of intra- and 
      inter-day were both less than 15%. The method can meet the determination 
      requirements of biological samples, and can be used for the determination of 
      mitomycin C in rabbit plasma after the administration of mitomycin C. The method 
      is selective, sensitive, convenient, rapid and reproducible in the determination 
      of mitomycin C, and also can be used for the pharmacokinetics research of 
      mitomycin C in plasma.
FAU - Tang, Yao
AU  - Tang Y
AD  - National Center for Safety Evaluation of Drugs, National Institutes for Food and 
      Drug Control, Beijing 100176, China.
FAU - Zhang, Shuangqing
AU  - Zhang S
FAU - Li, Xiang
AU  - Li X
FAU - Sun, Xu
AU  - Sun X
FAU - Wen, Nie
AU  - Wen N
FAU - Yu, Min
AU  - Yu M
FAU - Peng, Lili
AU  - Peng L
FAU - Li, Jinrang
AU  - Li J
FAU - Li, Zuogang
AU  - Li Z
FAU - Li, Bo
AU  - Li B
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 50SG953SK6 (Mitomycin)
RN  - F446C597KA (Triamcinolone Acetonide)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Mitomycin/*blood
MH  - Rabbits
MH  - Tandem Mass Spectrometry/*methods
MH  - Triamcinolone Acetonide/blood
EDAT- 2012/06/12 06:00
MHDA- 2013/07/16 06:00
CRDT- 2012/06/12 06:00
PHST- 2012/06/12 06:00 [entrez]
PHST- 2012/06/12 06:00 [pubmed]
PHST- 2013/07/16 06:00 [medline]
AID - 10.3724/sp.j.1123.2011.10034 [doi]
PST - ppublish
SO  - Se Pu. 2012 Feb;30(2):154-9. doi: 10.3724/sp.j.1123.2011.10034.