PMID- 22679838
OWN - NLM
STAT- MEDLINE
DCOM- 20130712
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 2
DP  - 2012 Feb
TI  - [Determination of cholesterol in drainage oil by solid phase extraction-gas 
      chromatography-mass spectrometry].
PG  - 207-10
AB  - An effective method was developed for the determination of cholesterol in 
      drainage oil by solid phase extraction-gas chromatography-mass spectrometry 
      (SPE-GC-MS). Firstly, the samples were purified by SPE on a column packed with 
      silica. An extraction yield of 97% was obtained when a 20 mL of ethyl 
      ether/n-hexane mixture (0.6: 99.4, v/v) was used for washing the SPE column, and 
      10 mL of the same solvent mixture (15: 85, v/v) for desorbing cholesterol from 
      the SPE column. Then the final extract was determined by GC-MS with electron 
      impact ion source. The cholesterol was characterized according to retention time 
      and characteristic fragments (m/z 213, 275, 301, 368, 386), and was 
      quantitatively determined by external standard method in selected ion monitoring 
      mode. The fragment of m/z 386 was selected as the target ion, and m/z 213 and m/z 
      275 were used as the reference ions. The average recovery was from 91.7% to 101%, 
      and the relative standard deviations (RSDs) were below 6%, and the detection 
      limit was 0.01 mg/L. A good linearity was obtained in the cholesterol 
      concentration range of 0.24 - 6.0 mg/L with the correlation coefficient of 0.999 
      6. The proposed method can be used to determine the cholesterol content in oil 
      accurately, and the detection result can be one of the criteria judging the 
      existence of drainage oil in edible oil.
FAU - Zhou, Yongsheng
AU  - Zhou Y
AD  - Institute of Petrochemical Technology, Changzhou University, Changzhou 213164, 
      China.
FAU - Luo, Shiping
AU  - Luo S
FAU - Kong, Yong
AU  - Kong Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Oils)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Cholesterol/*analysis
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Oils/*chemistry
MH  - Solid Phase Extraction/*methods
EDAT- 2012/06/12 06:00
MHDA- 2013/07/16 06:00
CRDT- 2012/06/12 06:00
PHST- 2012/06/12 06:00 [entrez]
PHST- 2012/06/12 06:00 [pubmed]
PHST- 2013/07/16 06:00 [medline]
AID - 10.3724/sp.j.1123.2011.12045 [doi]
PST - ppublish
SO  - Se Pu. 2012 Feb;30(2):207-10. doi: 10.3724/sp.j.1123.2011.12045.