PMID- 22679840
OWN - NLM
STAT- MEDLINE
DCOM- 20130712
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 2
DP  - 2012 Feb
TI  - [Extraction of inosinic acid in smoked Katsuwonus pelamis based on water].
PG  - 215-8
AB  - A novel method for the extraction of inosinic acid in Smoked Katsuwonus pelamis 
      based on water was developed. The inosinic acid was extracted by 95 degrees C 
      water for 5 min. The subsequent analysis of inosinic acid was achieved on an 
      Agilent Zorbax SB-C18 column (150 mm x 4.6 mm, 5 microm) with the mobile phase of 
      methanol-50 mmol/L ammonium acetate (5: 95, v/v) at a flow rate of 1.0 mL/min at 
      30 degrees C. The detection wavelength was set at 254 nm. The results showed that 
      the linear range for inosinic acid in Smoked Katsuwonus pelamis was between 1.0 
      mg/L and 120.0 mg/L with the limit of detection (LOD, S/N = 3) of 4 mg/kg and the 
      limit of quantification (LOQ, S/N = 10) of 12 mg/kg. At the spiked levels of 
      0.40, 2.00, 4.00 g/kg (n = 3), the recoveries ranged from 84.6% to 100.2% with 
      the relative standard deviations (RSDs) between 1.00% and 6.12%. Compared with 
      traditional extraction method by perchloric acid, the extraction efficiency was 
      improved by 37.0%. The novel method is safe, stable and accurate for the 
      determination of inosinic acid in Smoked Katsuwonus pelamis, which can also be 
      applied to the determination of inosinic acid in other foods and deserved to be 
      spread.
FAU - Wang, Tingting
AU  - Wang T
AD  - College of Chemical Engineering, Ningbo University of Technology, Ningbo 315016, 
      China. shiming-49@163.com
FAU - Chen, Yihui
AU  - Chen Y
FAU - Hu, Minjie
AU  - Hu M
FAU - Li, Xuezhen
AU  - Li X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 059QF0KO0R (Water)
RN  - 131-99-7 (Inosine Monophosphate)
SB  - IM
MH  - Chemical Fractionation/*methods
MH  - Fish Products/*analysis
MH  - Inosine Monophosphate/*analysis/*isolation & purification
MH  - Water
EDAT- 2012/06/12 06:00
MHDA- 2013/07/16 06:00
CRDT- 2012/06/12 06:00
PHST- 2012/06/12 06:00 [entrez]
PHST- 2012/06/12 06:00 [pubmed]
PHST- 2013/07/16 06:00 [medline]
AID - 10.3724/sp.j.1123.2011.10031 [doi]
PST - ppublish
SO  - Se Pu. 2012 Feb;30(2):215-8. doi: 10.3724/sp.j.1123.2011.10031.