PMID- 22683696
OWN - NLM
STAT- MEDLINE
DCOM- 20130205
LR  - 20200106
IS  - 1873-474X (Electronic)
IS  - 0736-5748 (Linking)
VI  - 30
IP  - 6
DP  - 2012 Oct
TI  - In vitro differentiation of human umbilical cord mesenchymal stem cells 
      (hUCMSCs), derived from Wharton's jelly, into choline acetyltransferase 
      (ChAT)-positive cells.
PG  - 471-7
LID - 10.1016/j.ijdevneu.2012.05.006 [doi]
AB  - We isolated and expanded fibroblast-like cells from the Wharton's jelly of human 
      umbilical cord successfully. Immunocytochemistry showed that they were positive 
      for several markers of mesenchymal stem cells (CD73, CD90, and CD105) and 
      integrin markers (CD29 and CD44), but negative for a hematopoietic cell maker 
      (CD45) and an endothelial cell marker (CD31). Their differentiation into 
      osteocytes and adipocytes under specific conditions indicated that they had 
      multi-lineage differentiation potential. Therefore these results proved that the 
      cells we obtained from Wharton's jelly were human umbilical cord mensenchymal 
      stem cells (hUCMSCs). Using immunocytochemistry and Western blotting analysis, we 
      found that after treatment with neuronal induction medium [NIM; consisting of 
      brain-derived neurotrophic factor (BDNF) and low-serum media] for 14 days, 
      hUCMSCs expressed a neuronal specific marker, microtubule associated protein 2 
      (MAP2), and extended neurite-like processes. After treatment with NIM, 
      supplemented with hippocampal cholinergic neurostimulating peptide (HCNP) or rat 
      denervated hippocampal extract [rDHE; derived from rat fimbria fornix (FF) 
      transected hippocampus], hUCMSCs expressed choline acetytransferase (ChAT) and 
      this action could be enhanced when cells were cultured with NIM, supplemented 
      with HCNP and rDHE in combination. ELISA showed that these ChAT-positive cells 
      could secrete acetylcholine (ACh). These findings indicate that hUCMSCs possess 
      the potential of differentiation into functional ChAT-positive cells in vitro and 
      provide a new candidate of cells for the cell transplantation to treat 
      Alzheimer's disease (AD).
CI  - Copyright (c) 2012 ISDN. Published by Elsevier Ltd. All rights reserved.
FAU - Zhang, Lei
AU  - Zhang L
AD  - Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of 
      Neuroregeneration, Nantong University, Nantong 226001, People's Republic of 
      China. zhanglei@ntu.edu.cn
FAU - Tan, Xuefeng
AU  - Tan X
FAU - Dong, Chuanming
AU  - Dong C
FAU - Zou, Linqing
AU  - Zou L
FAU - Zhao, Heyan
AU  - Zhao H
FAU - Zhang, Xinhua
AU  - Zhang X
FAU - Tian, Meiling
AU  - Tian M
FAU - Jin, Guohua
AU  - Jin G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120605
PL  - United States
TA  - Int J Dev Neurosci
JT  - International journal of developmental neuroscience : the official journal of the 
      International Society for Developmental Neuroscience
JID - 8401784
RN  - 0 (Antigens, CD)
RN  - 0 (Nerve Tissue Proteins)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/metabolism
MH  - Adipogenesis
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Cell Differentiation/drug effects/*physiology
MH  - Cells, Cultured
MH  - Choline O-Acetyltransferase/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Hippocampus/cytology
MH  - Humans
MH  - In Vitro Techniques
MH  - Male
MH  - Mesenchymal Stem Cells/*physiology
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurons/*enzymology
MH  - Osteogenesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Umbilical Cord/*anatomy & histology/cytology
MH  - Wharton Jelly/*cytology
EDAT- 2012/06/12 06:00
MHDA- 2013/02/06 06:00
CRDT- 2012/06/12 06:00
PHST- 2012/02/27 00:00 [received]
PHST- 2012/05/25 00:00 [revised]
PHST- 2012/05/25 00:00 [accepted]
PHST- 2012/06/12 06:00 [entrez]
PHST- 2012/06/12 06:00 [pubmed]
PHST- 2013/02/06 06:00 [medline]
AID - S0736-5748(12)00411-X [pii]
AID - 10.1016/j.ijdevneu.2012.05.006 [doi]
PST - ppublish
SO  - Int J Dev Neurosci. 2012 Oct;30(6):471-7. doi: 10.1016/j.ijdevneu.2012.05.006. 
      Epub 2012 Jun 5.