PMID- 22684116
OWN - NLM
STAT- MEDLINE
DCOM- 20121106
LR  - 20211021
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 30
IP  - 3
DP  - 2012 Sep
TI  - Establishment of immortalized Schwann cells derived from rat embryo dorsal root 
      ganglia.
PG  - 480-6
LID - 10.3892/ijmm.2012.1016 [doi]
AB  - Schwann cells (SCs) play an important role in the development, function and 
      regeneration of peripheral nerves. They can enhance both peripheral and central 
      nerve regeneration by providing a supportive environment for neurite outgrowth 
      through the release of neurotrophic factors. However, use of primary SCs for in 
      vitro models is limited because these cells are difficult to prepare and maintain 
      in high yield and purity under common cell culture conditions. Human telomerase 
      reverse transcriptase (hTERT) expression induces immortalization of various cell 
      types without substantial alterations of their phenotypes. Therefore, in this 
      study we transfected SCs with hTERT to establish a reliable cell source and 
      observed the effect of hTERT on SCs. In order to accomplish this, SCs were 
      isolated from rat embryo dorsal root ganglions, transfected with hTERT at early 
      passage (passage 3). SCs passage 4, 8, 12 and 30 after transfection (hTERT-SCs) 
      were used for immunocytochemistry, RT-PCR and western blotting. Results showed 
      that all the early (passage 4) and late (passage 30) passage hTERT-SCs expressed 
      hTERT mRNA and gained full telomerase activity. The transfection did not alter 
      the mRNA expression of senescence-associated genes, such as p53 and p16. The 
      expression of BDNF (brain-derived neurotrophic factor) was significantly 
      decreased as cell passage increased, compared to the untransfected control. On 
      the other hand, the expression of NGF (nerve growth factor ) was elevated at 
      early passages (passages 4 and 8) and decreased at late passages (12 and 30). 
      These data indicate that the use of specific immortalization techniques can 
      establish SC lines that retain characteristics of typical primary SCs, and 
      different mechanisms responsible for regulating NGF and BDNF expression. This is 
      the first report regarding the immortalization of SCs derived from rat embryo 
      dorsal root ganglions. These cells are useful in studies investigating the 
      cellular mechanisms and regenerative processes of SCs.
FAU - Jiang, Huajun
AU  - Jiang H
AD  - Department of Orthopaedics, First Affiliated Hospital of Dalian Medical 
      University, Dalian 116011, P.R. China.
FAU - Qu, Wei
AU  - Qu W
FAU - Han, Feng
AU  - Han F
FAU - Liu, Dazhuang
AU  - Liu D
FAU - Zhang, Weiguo
AU  - Zhang W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120606
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - *Cell Line
MH  - Cyclin-Dependent Kinase Inhibitor p16/genetics/metabolism
MH  - Enzyme Activation
MH  - Female
MH  - Ganglia, Spinal/*cytology/embryology
MH  - Gene Expression
MH  - Gene Expression Regulation
MH  - Humans
MH  - Nerve Growth Factor/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Schwann Cells/*cytology/metabolism
MH  - Telomerase/*genetics/metabolism
MH  - Transfection
MH  - Tumor Suppressor Protein p53/genetics/metabolism
PMC - PMC3573738
EDAT- 2012/06/12 06:00
MHDA- 2012/11/07 06:00
PMCR- 2012/06/06
CRDT- 2012/06/12 06:00
PHST- 2012/03/02 00:00 [received]
PHST- 2012/04/30 00:00 [accepted]
PHST- 2012/06/12 06:00 [entrez]
PHST- 2012/06/12 06:00 [pubmed]
PHST- 2012/11/07 06:00 [medline]
PHST- 2012/06/06 00:00 [pmc-release]
AID - ijmm-30-03-0480 [pii]
AID - 10.3892/ijmm.2012.1016 [doi]
PST - ppublish
SO  - Int J Mol Med. 2012 Sep;30(3):480-6. doi: 10.3892/ijmm.2012.1016. Epub 2012 Jun 
      6.