PMID- 22684550
OWN - NLM
STAT- MEDLINE
DCOM- 20140911
LR  - 20211021
IS  - 1672-0733 (Print)
IS  - 1672-0733 (Linking)
VI  - 32
IP  - 3
DP  - 2012 Jun
TI  - Inhibition of HBV replication by VPS4B and its dominant negative mutant 
      VPS4B-K180Q in vivo.
PG  - 311-316
LID - 10.1007/s11596-012-0054-2 [doi]
AB  - This study examined the anti-hepatitis B virus (HBV) effect of wild-type (WT) 
      vacuolar protein sorting 4B (VPS4B) and its dominant negative (DN) mutant 
      VPS4B-K180Q in vivo in order to further explore the relationship between HBV and 
      the host cellular factor VPS4. VPS4B gene was amplified from Huh7 cells by RT-PCR 
      and cloned into the eukaryotic expression vector pXF3H. Then, the VPS4B plasmid 
      and the VPS4B-K180Q mutation plasmid were constructed by using the overlap 
      extension PCR site-directed mutagenesis technique. VPS4B and HBV vectors were 
      co-delivered into mice by the hydrodynamic tail-vein injection to establish HBV 
      vector-based models. Quantities of HBsAg and HBeAg in the mouse sera were 
      determined by ElectroChemiLuminescence (ECL). HBV DNA in sera was measured by 
      real-time quantitative PCR. Southern blot analysis was used to assay the 
      intracellular HBV nuclear capsid-related DNA, real-time quantitative PCR to 
      detect the HBV-related mRNA and immunohistochemical staining to observe the HBcAg 
      expression in the mouse liver tissues. Our results showed that VPS4B and its 
      mutant VPS4B-K180Q could decrease the levels of serum HBsAg, HBeAg and HBV-DNA. 
      In addition, the HBV DNA replication and the mRNA level of HBV in the liver 
      tissues of treated mice could be suppressed by VPS4B and VPS4B-K180Q. It was also 
      found that VPS4B and VPS4B-K180Q had an ability to inhibit core antigen 
      expression in the infected mouse liver. Furthermore, the anti-HBV effect of 
      mutant VPS4B-K180Q was more potent than that of wild-type VPS4B. Taken together, 
      it was concluded that VPS4B and its DN mutant VPS4B-K180Q have anti-HBV effect in 
      vivo, which helps develop molecular therapeutic strategies for HBV infection.
FAU - Xia, Jianbo
AU  - Xia J
AD  - Division of Clinical Immunology, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, 430030, China.
AD  - Department of Clinical Laboratory, Hubei Maternal and Child Health Hospital, 
      Wuhan, 430070, China.
FAU - Wang, Weipeng
AU  - Wang W
AD  - Division of Clinical Immunology, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, 430030, China.
AD  - Department of Clinical Laboratory, Hubei Maternal and Child Health Hospital, 
      Wuhan, 430070, China.
FAU - Li, Lei
AU  - Li L
AD  - Department of Infectious Disease, Anhui Provincial Hospital, Hefei, 230001, 
      China.
FAU - Liu, Zhi
AU  - Liu Z
AD  - Department of Clinical Laboratory, Hubei Maternal and Child Health Hospital, 
      Wuhan, 430070, China.
FAU - Liu, Min
AU  - Liu M
AD  - Division of Clinical Immunology, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, 430030, China.
FAU - Yang, Dongliang
AU  - Yang D
AD  - Division of Clinical Immunology, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, 430030, China. 
      dlyang@tjh.tjmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120609
PL  - China
TA  - J Huazhong Univ Sci Technolog Med Sci
JT  - Journal of Huazhong University of Science and Technology. Medical sciences = Hua 
      zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. 
      Yixue Yingdewen ban
JID - 101169627
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.4.- (ATPases Associated with Diverse Cellular Activities)
RN  - EC 3.6.4.6 (VPS4B protein, human)
SB  - IM
MH  - ATPases Associated with Diverse Cellular Activities
MH  - Adenosine Triphosphatases/*physiology
MH  - Animals
MH  - Endosomal Sorting Complexes Required for Transport/*physiology
MH  - Female
MH  - Genes, Dominant/genetics
MH  - Hepatitis B/*metabolism/*virology
MH  - Hepatitis B virus/*physiology
MH  - Liver/*virology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mutation/genetics
MH  - Virus Inactivation
EDAT- 2012/06/12 06:00
MHDA- 2014/09/12 06:00
CRDT- 2012/06/12 06:00
PHST- 2011/07/13 00:00 [received]
PHST- 2012/06/12 06:00 [entrez]
PHST- 2012/06/12 06:00 [pubmed]
PHST- 2014/09/12 06:00 [medline]
AID - 10.1007/s11596-012-0054-2 [pii]
AID - 10.1007/s11596-012-0054-2 [doi]
PST - ppublish
SO  - J Huazhong Univ Sci Technolog Med Sci. 2012 Jun;32(3):311-316. doi: 
      10.1007/s11596-012-0054-2. Epub 2012 Jun 9.