PMID- 22685355
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20120824
LR  - 20211021
IS  - 1347-5800 (Electronic)
IS  - 0044-5991 (Print)
IS  - 0044-5991 (Linking)
VI  - 45
IP  - 2
DP  - 2012 Apr 26
TI  - Immunocytochemical colocalization of fibroblast growth factor-1 with 
      neurotrophin-3 in mouse alveolar macrophages.
PG  - 131-7
LID - 10.1267/ahc.11055 [doi]
AB  - Alveolar macrophages are known to express a variety of growth factors and 
      neurotrophins. Fibroblast growth factor-1 (FGF-1) is abundantly present in the 
      lung and has mitogenic and neurotrophic activities similarly to neurotrophins. In 
      order to determine whether FGF-1 associates with neurotrophins in alveolar 
      macrophages, we investigated the immunocytochemical colocalization of FGF-1 with 
      neurotrophins, nerve growth factor (NGF), brain-derived neurotrophic factor 
      (BDNF), and neurotrophin-3 (NT-3), in mouse alveolar macrophages. The results 
      showed that 34% of macrophages were immunoreactive for FGF-1, 10% for NGF, 9% for 
      BDNF, and 17% for NT-3. Of FGF-1-immunoreactive (IR) macrophages, 16% were 
      immunoreactive for NT-3, but only small percentages were immunoreactive for NGF 
      (0.8%) and for BDNF (0.3%). FGF-1 and neurotrophins were all localized in the 
      intracellular vesicles. In the vesicles, FGF-1 and NT-3 were frequently 
      colocalized. All macrophages expressed lysosome-associated protein-2 (LAMP-2), a 
      late endosomal and lysosomal marker, and early endosomes antigen 1 (EEA1), an 
      early endosomal marker. FGF-1 and NT-3 were predominantly colocalized with LAMP-2 
      rather than with EEA1, whereas NGF and BDNF were colocalized with EEA1 rather 
      than with LAMP-2. These results indicate that FGF-1 and NT-3 are substantially 
      expressed in mouse alveolar macrophages and colocalized in vesicles, 
      predominantly in late endosomes and lysosomes.
FAU - Hiruma, Hiromi
AU  - Hiruma H
AD  - Department of Physiology, Kitasato University School of Medicine, 1-15-1 
      Kitasato, Minami-ku, Sagamihara, 252-0374, Japan.
FAU - Hikawa, Shiori
AU  - Hikawa S
FAU - Kawakami, Tadashi
AU  - Kawakami T
LA  - eng
PT  - Journal Article
DEP - 20120314
PL  - Japan
TA  - Acta Histochem Cytochem
JT  - Acta histochemica et cytochemica
JID - 0147110
PMC - PMC3365304
OTO - NOTNLM
OT  - BDNF
OT  - FGF-1
OT  - NGF
OT  - NT-3
OT  - mouse alveolar macrophages
EDAT- 2012/06/12 06:00
MHDA- 2012/06/12 06:01
PMCR- 2012/04/26
CRDT- 2012/06/12 06:00
PHST- 2011/11/07 00:00 [received]
PHST- 2012/02/02 00:00 [accepted]
PHST- 2012/06/12 06:00 [entrez]
PHST- 2012/06/12 06:00 [pubmed]
PHST- 2012/06/12 06:01 [medline]
PHST- 2012/04/26 00:00 [pmc-release]
AID - AHC11055 [pii]
AID - 10.1267/ahc.11055 [doi]
PST - ppublish
SO  - Acta Histochem Cytochem. 2012 Apr 26;45(2):131-7. doi: 10.1267/ahc.11055. Epub 
      2012 Mar 14.