PMID- 22687405
OWN - NLM
STAT- MEDLINE
DCOM- 20121015
LR  - 20190720
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 35
IP  - 5
DP  - 2012
TI  - Mizoribine suppresses proliferation of rat glomerular epithelial cells in culture 
      and inhibits increase of monocyte chemoattractant protein-1 and macrophage 
      inflammatory protein-2 stimulated by thrombin.
PG  - 705-8
AB  - Glomerular crescents play an important role in progressive glomerular injury. The 
      lesions consist of epithelial cells, macrophages and fibrin deposition. 
      Macrophage chemoattractant protin-1 (MCP-1) is a chemoattractant of monocytes, 
      which has a potential of procoagulant activity. Macrophage inflammatory protein-2 
      (MIP-2) is a chemoattractant of neutrophils and acute necrotizing injury is 
      primarily mediated by neutrophils in crescentic glomerulonephritis. Mizoribine 
      (MZR) is an immunosuppressive drug and it has been used for organ transplantation 
      and treatment of various autoimmune diseases. The aim of this study is to 
      investigate the effects of MZR on glomerular epithelial cells (GEC). Rat GEC were 
      cultured with K1 medium and used from 12th to 14th passage. GEC proliferation was 
      determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) 
      assay. MCP-1 and MIP-2 were quantified by enzyme-linked immunosorbent assay 
      (ELISA) in culture supernatants and mRNA expressions of MCP-1 and MIP-2 were 
      analyzed by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). 
      The proliferation of GEC was suppressed by MZR in a dose-dependent manner in the 
      range of 1.0-100.0 microg/mL. These concentrations of MZR had no toxic effect to GEC. 
      Thrombin (1.0-5.0 U/mL) enhanced the production of MCP-1, MIP-2 and the mRNA 
      expressions of MCP-1 and MIP-2. The stimulatory effect of thrombin was inhibited 
      by addition of MZR (10 microg/mL). It is concluded that MZR may be useful for the 
      treatment of crescentic glomerulonephritis.
FAU - Yamabe, Hideaki
AU  - Yamabe H
AD  - Department of Nephrology, Graduate School of Medicine, Hirosaki University, 
      Japan. yamabe@cc.hirosaki-u.ac.jp
FAU - Shimada, Michiko
AU  - Shimada M
FAU - Murakami, Reiichi
AU  - Murakami R
FAU - Fujita, Takeshi
AU  - Fujita T
FAU - Shimaya, Yuko
AU  - Shimaya Y
FAU - Nakamura, Norio
AU  - Nakamura N
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (RNA, Messenger)
RN  - 0 (Ribonucleosides)
RN  - 4JR41A10VP (mizoribine)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology/therapeutic use
MH  - Cattle
MH  - Cell Proliferation/*drug effects
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Chemokine CXCL2/genetics/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Epithelial Cells/cytology/*drug effects/metabolism
MH  - Glomerulonephritis/drug therapy/metabolism/pathology
MH  - Immunosuppressive Agents/pharmacology/therapeutic use
MH  - Kidney Glomerulus/cytology/*drug effects/metabolism
MH  - Male
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Ribonucleosides/*pharmacology/therapeutic use
MH  - Thrombin/*pharmacology
EDAT- 2012/06/13 06:00
MHDA- 2012/10/16 06:00
CRDT- 2012/06/13 06:00
PHST- 2012/06/13 06:00 [entrez]
PHST- 2012/06/13 06:00 [pubmed]
PHST- 2012/10/16 06:00 [medline]
AID - JST.JSTAGE/bpb/35.705 [pii]
AID - 10.1248/bpb.35.705 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2012;35(5):705-8. doi: 10.1248/bpb.35.705.