PMID- 22687713
OWN - NLM
STAT- MEDLINE
DCOM- 20121113
LR  - 20131121
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1252
DP  - 2012 Aug 24
TI  - Repeatability of the efficiency of columns packed with sub-3mum core-shell 
      particles: Part II. 2.7 mum Halo-ES-Peptide-C18 particles in 4.6mm and 2.1mmx100mm 
      column formats.
PG  - 45-55
LID - 10.1016/j.chroma.2012.05.063 [doi]
AB  - The column-to-column repeatability of the mass transfer kinetics in columns 
      packed with sub-3mum shell particles was investigated. The parameters of this 
      kinetics were measured for twelve columns (six 2.1mmx100mm and six 4.6mmx100mm) 
      packed with the same batch of 2.7mum Halo-ES-Peptide-C(18) particles (Advanced 
      Material Technologies, Wilmington, DE, USA). For both series, the manufacturer 
      provided columns at different positions in the efficiency distribution given by 
      the quality test control. Three compounds were used, uracil, naphthalene and 
      insulin. The reduced longitudinal diffusion term was measured with the peak 
      parking (PP) method; the reduced solid-liquid mass transfer resistance term was 
      given by a combination of the PP results and the most accurate model of effective 
      diffusion in ternary composite materials (non-porous cores, concentric porous 
      shell, and eluent matrix), validated previously. The overall eddy diffusion term 
      was obtained by subtraction of these two HETP terms from the overall reduced HETP 
      measured by numerical integration of the entire peak profiles. The results 
      demonstrate that the dispersion of the column efficiencies is mostly due to the 
      random nature of the packing process and the associated eddy diffusion term. At 
      the highest reduced velocity achieved, the relative standard deviations (RSDs) of 
      the eddy diffusion term for the 2.1mm I.D. columns were ca. 5 and 10% (with 
      average values A(nu)=2.3 and 8.5) for naphthalene and uracil, respectively. For 
      the 4.6mm I.D. columns, these RSDs were 3 and 5%, respectively, with average 
      values A(nu)=1.5 and 2.7.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Gritti, Fabrice
AU  - Gritti F
AD  - Department of Chemistry, University of Tennessee, Knoxville, TN 37996-1600, USA.
FAU - Guiochon, Georges
AU  - Guiochon G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120531
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Insulin)
RN  - 0 (Naphthalenes)
RN  - 2166IN72UN (naphthalene)
RN  - 56HH86ZVCT (Uracil)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*instrumentation/*methods
MH  - Diffusion
MH  - Insulin/chemistry
MH  - Kinetics
MH  - *Microspheres
MH  - Models, Chemical
MH  - Naphthalenes/chemistry
MH  - Particle Size
MH  - Permeability
MH  - Reproducibility of Results
MH  - Temperature
MH  - Uracil/chemistry
EDAT- 2012/06/13 06:00
MHDA- 2012/11/14 06:00
CRDT- 2012/06/13 06:00
PHST- 2012/04/11 00:00 [received]
PHST- 2012/05/15 00:00 [revised]
PHST- 2012/05/16 00:00 [accepted]
PHST- 2012/06/13 06:00 [entrez]
PHST- 2012/06/13 06:00 [pubmed]
PHST- 2012/11/14 06:00 [medline]
AID - S0021-9673(12)00792-3 [pii]
AID - 10.1016/j.chroma.2012.05.063 [doi]
PST - ppublish
SO  - J Chromatogr A. 2012 Aug 24;1252:45-55. doi: 10.1016/j.chroma.2012.05.063. Epub 
      2012 May 31.