PMID- 22687753 OWN - NLM STAT- MEDLINE DCOM- 20121119 LR - 20191210 IS - 1873-2399 (Electronic) IS - 0301-472X (Linking) VI - 40 IP - 10 DP - 2012 Oct TI - SPARC promotes the development of erythroid progenitors. PG - 828-36 LID - S0301-472X(12)00228-7 [pii] LID - 10.1016/j.exphem.2012.06.002 [doi] AB - Secreted protein acidic and rich in cysteine (SPARC) is a well-recognized regulator that affects cell proliferation, differentiation, and survival. Here, we investigated the impact of SPARC on erythropoiesis. Erythropoiesis in bone marrow (BM) from SPARC(-/-) mice was analyzed by flow cytometry and colony-forming assays. The mechanisms that affected erythropoiesis were investigated by BM transplantation experiments. CD34(+) cells from umbilical cord blood were isolated by MiniMACS, and the effect of SPARC on human erythropoiesis was assessed by colony-formation assay and erythroid differentiation culture. The hemoglobin level in peripheral blood (PB) was lower in SPARC(-/-) mice. Neither red blood cell count in PB nor the percentage of Ter119(+) erythrocytes in BM showed significant difference between SPARC(-/-) and WT mice. However, the ability of SPARC(-/-) bone marrow cells (BMCs) to form erythroid burst-forming units (BFU-E), as well as spleen colony-forming units (CFU-S8), was significantly decreased. The addition of exogenous SPARC could promote the formation of BFU-E from SPARC(-/-) BMCs in vitro. And the impaired ability of SPARC(-/-) BMCs to form BFU-E could be restored by transplanted into WT BM microenvironment, whereas the BFU-E formation of WT BMCs was impaired after transplanted into SPARC(-/-) recipients. Furthermore, exogenous SPARC promoted umbilical cord blood CD34(+) cells to form erythroid colonies and the hemoglobinization of erythroid, but did not affect the expression levels of glycophorin A and CD71. In conclusion, our results indicate that SPARC promotes the development of erythroid progenitors, but does not affect terminal erythroid differentiation. CI - Copyright (c) 2012 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc. All rights reserved. FAU - Luo, Zhen AU - Luo Z AD - Department of Adult Stem Cells, Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha, China. FAU - Luo, Pan AU - Luo P FAU - Yu, Yan AU - Yu Y FAU - Zhao, Qian AU - Zhao Q FAU - Zhao, Xiuhua AU - Zhao X FAU - Cheng, Lamei AU - Cheng L LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20120609 PL - Netherlands TA - Exp Hematol JT - Experimental hematology JID - 0402313 RN - 0 (Antigens, CD) RN - 0 (CD71 antigen) RN - 0 (Glycophorins) RN - 0 (Osteonectin) RN - 0 (Receptors, Transferrin) SB - IM MH - Animals MH - Antigens, CD/genetics/metabolism MH - Bone Marrow Transplantation MH - Cell Differentiation/*physiology MH - Cells, Cultured MH - Colony-Forming Units Assay/methods MH - Erythroid Precursor Cells/cytology/*metabolism MH - Erythropoiesis/*physiology MH - Fetal Blood/cytology/*metabolism MH - Glycophorins/genetics/metabolism MH - Humans MH - Mice MH - Mice, Knockout MH - Osteonectin/genetics/*metabolism MH - Receptors, Transferrin/genetics/metabolism EDAT- 2012/06/13 06:00 MHDA- 2012/12/10 06:00 CRDT- 2012/06/13 06:00 PHST- 2012/04/09 00:00 [received] PHST- 2012/05/26 00:00 [revised] PHST- 2012/06/05 00:00 [accepted] PHST- 2012/06/13 06:00 [entrez] PHST- 2012/06/13 06:00 [pubmed] PHST- 2012/12/10 06:00 [medline] AID - S0301-472X(12)00228-7 [pii] AID - 10.1016/j.exphem.2012.06.002 [doi] PST - ppublish SO - Exp Hematol. 2012 Oct;40(10):828-36. doi: 10.1016/j.exphem.2012.06.002. Epub 2012 Jun 9.