PMID- 22688982
OWN - NLM
STAT- MEDLINE
DCOM- 20130729
LR  - 20201226
IS  - 0717-6287 (Electronic)
IS  - 0716-9760 (Linking)
VI  - 45
IP  - 1
DP  - 2012
TI  - Human dendritic cells sequentially matured with CD4(+) T cells as a secondary 
      signal favor CTL and long-term T memory cell responses.
PG  - 33-43
LID - S0716-97602012000100005 [pii]
LID - 10.4067/S0716-97602012000100005 [doi]
AB  - Dendritic cells (DCs) are professional antigen-presenting cells involved in the 
      control and initiation of immune responses. In vivo, DCs exposed at the periphery 
      to maturation stimuli migrate to lymph nodes, where they receive secondary 
      signals from CD4+ T helper cells. These DCs become able to initiate CD8+ 
      cytotoxic T lymphocyte (CTL) responses. However, in vitro investigations 
      concerning human monocyte-derived DCs have never focused on their functional 
      properties after such sequential maturation. Here, we studied human DC phenotypes 
      and functions according to this sequential exposure to maturation stimuli. As 
      first signals, we used TNF-alpha/polyI:C mimicking inflammatory and pathogen stimuli 
      and, as second signals, we compared activated CD4+ T helper cells to a 
      combination of CD40-L/ IFN-gamma. Our results show that a sequential activation with 
      activated CD4+ T cells dramatically increased the maturation of DCs in terms of 
      their phenotype and cytokine secretion compared to DCs activated with maturation 
      stimuli delivered simultaneously. Furthermore, this sequential maturation led to 
      the induction of CTL with a long-term effector and central memory phenotypes. 
      Thus, sequential delivery of maturation stimuli, which includes CD4+ T cells, 
      should be considered in the future to improve the induction of long-term CTL 
      memory in DC-based immunotherapy.
FAU - Simon, Thomas
AU  - Simon T
AD  - Institut de Recherche Therapeutique, Universite de Nantes, Nantes, France. 
      marc.gregoire@nantes.inserm.fr
FAU - Tanguy-Royer, Severine
AU  - Tanguy-Royer S
FAU - Royer, Pierre-Joseph
AU  - Royer PJ
FAU - Boisgerault, Nicolas
AU  - Boisgerault N
FAU - Frikeche, Jihane
AU  - Frikeche J
FAU - Fonteneau, Jean-Francois
AU  - Fonteneau JF
FAU - Gregoire, Marc
AU  - Gregoire M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biol Res
JT  - Biological research
JID - 9308271
RN  - 0 (CD4 Antigens)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - CD4 Antigens/*analysis
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*immunology
MH  - Humans
MH  - Immunologic Memory/*immunology
MH  - Immunophenotyping
MH  - Immunotherapy
MH  - Interferon-gamma/immunology
MH  - Lymphocyte Activation
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - T-Lymphocytes, Helper-Inducer/*immunology
MH  - Tumor Necrosis Factor-alpha/immunology
EDAT- 2012/06/13 06:00
MHDA- 2013/07/31 06:00
CRDT- 2012/06/13 06:00
PHST- 2011/06/09 00:00 [received]
PHST- 2011/11/10 00:00 [accepted]
PHST- 2012/06/13 06:00 [entrez]
PHST- 2012/06/13 06:00 [pubmed]
PHST- 2013/07/31 06:00 [medline]
AID - S0716-97602012000100005 [pii]
AID - 10.4067/S0716-97602012000100005 [doi]
PST - ppublish
SO  - Biol Res. 2012;45(1):33-43. doi: 10.4067/S0716-97602012000100005.