PMID- 22689490
OWN - NLM
STAT- MEDLINE
DCOM- 20121009
LR  - 20191210
IS  - 1615-9314 (Electronic)
IS  - 1615-9306 (Linking)
VI  - 35
IP  - 9
DP  - 2012 May
TI  - Capillary electrochromatography of three beta2-agonists in human urine using a 
      lauryl methacrylate-based monolithic column.
PG  - 1138-45
LID - 10.1002/jssc.201101005 [doi]
AB  - A new method of online concentration capillary electrochromatography using a 
      lauryl methacrylate-based monolithic column was developed for determination of 
      three beta(2)-agonists including salbutamol, procaterol, and formoterol. The 
      separation parameters including acetonitrile concentration, running buffer pH, 
      and concentration were evaluated. To improve the sensitivity, an online 
      concentration method with combination of the chromatographic zone-sharpening 
      effect and field-enhanced sample-stacking effect has been developed in which the 
      concentration parameters including injection voltage, injection time, as well as 
      sample matrix were systematically studied. Under the optimized conditions, 
      baseline separation of three beta(2)-agonists was achieved within 4 min. When 
      compared to the conventional sample injection, this online concentration 
      technique increased their corresponding sensitivities up to 45-, 36-, and 
      320-fold, respectively. Furthermore, good repeatability was obtained with 
      relative standard deviations (RSDs) for migration times within 0.84% and those 
      for peak areas less than 6.35% (n = 5) in the experiment. The proposed method was 
      successfully applied to the determination of above-mentioned beta(2)-agonists in 
      urine sample. The recoveries of spiked urine samples were between 82.4% and 
      109.1% with RSDs less than 9.97%.
CI  - (c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Chen, Zongbao
AU  - Chen Z
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Department of Chemistry, Fuzhou University, Fuzhou, Fujian, China.
FAU - Cai, Yan
AU  - Cai Y
FAU - Zhang, Lin
AU  - Zhang L
FAU - Zhang, Lan
AU  - Zhang L
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Sep Sci
JT  - Journal of separation science
JID - 101088554
RN  - 0 (Adrenergic beta-2 Receptor Agonists)
RN  - 0 (Ethanolamines)
RN  - QF8SVZ843E (Albuterol)
RN  - W34SHF8J2K (Formoterol Fumarate)
RN  - X7I3EMM5K0 (Procaterol)
SB  - IM
MH  - Adrenergic beta-2 Receptor Agonists/*urine
MH  - Albuterol/*urine
MH  - Capillary Electrochromatography/instrumentation/*methods
MH  - Ethanolamines/*urine
MH  - Formoterol Fumarate
MH  - Humans
MH  - Procaterol/*urine
EDAT- 2012/06/13 06:00
MHDA- 2012/10/10 06:00
CRDT- 2012/06/13 06:00
PHST- 2012/06/13 06:00 [entrez]
PHST- 2012/06/13 06:00 [pubmed]
PHST- 2012/10/10 06:00 [medline]
AID - 10.1002/jssc.201101005 [doi]
PST - ppublish
SO  - J Sep Sci. 2012 May;35(9):1138-45. doi: 10.1002/jssc.201101005.