PMID- 22691185
OWN - NLM
STAT- MEDLINE
DCOM- 20121119
LR  - 20220310
IS  - 1440-1827 (Electronic)
IS  - 1320-5463 (Linking)
VI  - 62
IP  - 7
DP  - 2012 Jul
TI  - Detection of kinase amplifications in gastric cancer archives using fluorescence 
      in situ hybridization.
PG  - 477-84
LID - 10.1111/j.1440-1827.2012.02832.x [doi]
AB  - To test the feasibility of using bacterial artificial chromosomes (BAC) 
      containing kinases for pathological diagnosis using fluorescence in situ 
      hybridization (FISH), 10 BAC probes containing a gene amplified in 5% or more of 
      a pilot cohort were selected from a previous survey using arbitrarily selected 
      BAC clones harboring 100 kinases. In this report, we describe the prevalence and 
      association with the clinicopathological profile of these selected 10 BAC probes 
      in 365 gastric cancer tissues. FISH analyses using these 10 BAC probes containing 
      loci encoding EGFR, ERBB2(HER2), EPHB3, PIK3CA, MET, PTK7, ACK1, STK15, SRC, and 
      HCK showed detectable amplifications in paraffin-embedded tissue in 2.83% to 
      13.6% of the gastric cancer tissues. Considerable numbers of the cases showed the 
      co-amplification of two or more of the probes that were tested. BAC probes 
      located within a genome neighborhood, such as PIK3CA, EPHB3, and ACK1 at 3q26-29 
      or HCK, SRC, and STK15 at 20q11-13.1, were often co-amplified in the same cases, 
      but non-random co-amplifications of genes at distant genomic loci were also 
      observed. These findings provide basic information regarding the creation of a 
      strategy for personalizing gastric cancer therapy, especially when using multiple 
      kinase inhibitors.
FAU - Kiyose, Shin-ichiro
AU  - Kiyose S
AD  - Department of Tumor Pathology, Hamamatsu University School of Medicine, 
      Higashi-ku, Hamamatsu, Japan.
FAU - Nagura, Kiyoko
AU  - Nagura K
FAU - Tao, Hong
AU  - Tao H
FAU - Igarashi, Hisaki
AU  - Igarashi H
FAU - Yamada, Hidetaka
AU  - Yamada H
FAU - Goto, Masanori
AU  - Goto M
FAU - Maeda, Matsuyoshi
AU  - Maeda M
FAU - Kurabe, Nobuya
AU  - Kurabe N
FAU - Suzuki, Masaya
AU  - Suzuki M
FAU - Tsuboi, Masaru
AU  - Tsuboi M
FAU - Kahyo, Tomoaki
AU  - Kahyo T
FAU - Shinmura, Kazuya
AU  - Shinmura K
FAU - Hattori, Naohiko
AU  - Hattori N
FAU - Sugimura, Haruhiko
AU  - Sugimura H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Pathol Int
JT  - Pathology international
JID - 9431380
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Protein Kinase Inhibitors)
RN  - EC 2.7.- (Protein Kinases)
SB  - IM
MH  - Adenocarcinoma/*genetics/secondary
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antineoplastic Agents/therapeutic use
MH  - Biological Specimen Banks
MH  - Chromosomes, Artificial, Bacterial
MH  - Cohort Studies
MH  - Feasibility Studies
MH  - Female
MH  - Gene Amplification/*genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Precision Medicine
MH  - Protein Kinase Inhibitors/therapeutic use
MH  - Protein Kinases/*genetics
MH  - Stomach Neoplasms/*genetics/pathology
MH  - Tissue Array Analysis
EDAT- 2012/06/14 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/06/14 06:00
PHST- 2012/06/14 06:00 [entrez]
PHST- 2012/06/14 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 10.1111/j.1440-1827.2012.02832.x [doi]
PST - ppublish
SO  - Pathol Int. 2012 Jul;62(7):477-84. doi: 10.1111/j.1440-1827.2012.02832.x.