PMID- 22691809
OWN - NLM
STAT- MEDLINE
DCOM- 20121214
LR  - 20131121
IS  - 1522-9629 (Electronic)
IS  - 1094-5539 (Linking)
VI  - 25
IP  - 4
DP  - 2012 Aug
TI  - Dexamethasone down-regulates the inflammatory mediators but fails to reduce the 
      tissue injury in the lung of acute pancreatitis rat models.
PG  - 319-24
LID - 10.1016/j.pupt.2012.05.009 [doi]
AB  - Pulmonary complications are frequent in the course of acute pancreatitis. We 
      investigate the effects of dexamethasone on lung injury in mild and severe AP. 
      Mild and severe acute pancreatitis was induced in rats by bile-pancreatic duct 
      obstruction and infusion of 3.5% sodium taurocholate into the bile-pancreatic 
      duct, respectively. Dexamethasone (1 mg/kg) was given by intramuscular injection 
      1 h after acute pancreatitis. Plasma amylase activity was measured to evaluate 
      the pancreas damage. Lungs were harvested for analysing mRNA expression of 
      monocyte chemoattractant protein-1 (MCP-1), cytokine-induced neutrophil 
      chemoattractant (CINC), P-selectin and intercellular adhesion molecule-1 
      (ICAM-1), myeloperoxidase (MPO) activity (as index of neutrophil infiltration) 
      and histological examination. Dexamethasone reduced the hyperamylasemia and 
      hindered the pulmonary upregulation of MCP-1, CINC, P-selectin and ICAM-1, in 
      both mild and severe acute pancreatitis. Despite this, dexamethasone treatment 
      failed to reduce MPO activity and histological alterations developed in lungs 
      during acute pancreatitis, either in bile-pancreatic duct obstruction or sodium 
      taurocholate model. We conclude that pulmonary local factors different from 
      inflammatory mediators contribute to leukocyte recruitment, so that although 
      dexamethasone down-regulated the lung expression of chemokines and adhesion 
      molecules during acute pancreatitis it was not able to prevent leukocyte 
      infiltration, which could be responsible for maintaining the lung injury in 
      either mild or severe acute pancreatitis.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Yubero, Sara
AU  - Yubero S
AD  - Department of Physiology and Pharmacology, IBSAL (Instituto Biosanitario 
      Salamanca), University of Salamanca, 37007 Salamanca, Spain.
FAU - Manso, Manuel A
AU  - Manso MA
FAU - Ramudo, Laura
AU  - Ramudo L
FAU - Vicente, Secundino
AU  - Vicente S
FAU - De Dios, Isabel
AU  - De Dios I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120609
PL  - England
TA  - Pulm Pharmacol Ther
JT  - Pulmonary pharmacology & therapeutics
JID - 9715279
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Chemokines)
RN  - 0 (RNA, Messenger)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 3.2.1.- (Amylases)
SB  - IM
MH  - Acute Disease
MH  - Acute Lung Injury/etiology/*physiopathology
MH  - Amylases/blood
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Cell Adhesion
MH  - Chemokines/immunology
MH  - Dexamethasone/*pharmacology
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Lung/immunology
MH  - Male
MH  - Pancreatitis/complications
MH  - RNA, Messenger
MH  - Rats
MH  - Rats, Wistar
MH  - Severity of Illness Index
EDAT- 2012/06/14 06:00
MHDA- 2012/12/15 06:00
CRDT- 2012/06/14 06:00
PHST- 2012/03/20 00:00 [received]
PHST- 2012/05/30 00:00 [revised]
PHST- 2012/05/31 00:00 [accepted]
PHST- 2012/06/14 06:00 [entrez]
PHST- 2012/06/14 06:00 [pubmed]
PHST- 2012/12/15 06:00 [medline]
AID - S1094-5539(12)00073-9 [pii]
AID - 10.1016/j.pupt.2012.05.009 [doi]
PST - ppublish
SO  - Pulm Pharmacol Ther. 2012 Aug;25(4):319-24. doi: 10.1016/j.pupt.2012.05.009. Epub 
      2012 Jun 9.