PMID- 22692751
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20141212
IS  - 1833-3516 (Print)
IS  - 1833-3516 (Linking)
VI  - 38
IP  - 4
DP  - 2008 Dec
TI  - Differential effect of high pressure on NMDA receptor currents in Xenopus laevis 
      oocytes.
PG  - 194-6
AB  - Hyperbaric environments over 1.1 MPa induce in mammals and humans the high 
      pressure neurological syndrome (HPNS). HPNS is characterized by cognitive and 
      motor decrements associated with sleep disorders, EEG changes, tremor, and 
      convulsions. Previously, it was proposed that augmented responses of the 
      glutamatergic N-methyl-D-aspartate receptor (NMDAR) may be involved. Recently, we 
      have reported that, in rat hippocampus brain slices, isolated NMDAR synaptic 
      responses were augmented at high pressure. We now test how high pressure affects 
      NMDAR ionic currents. Mammalian (rat) cRNA of the most common hippocampal NMDAR 
      compositions NR1-1a / NR2A and NR1-1b / NR2A were injected into Xenopus laevis 
      oocytes, and NMDAR ionic currents were recorded, applying the two-electrode 
      voltage clamp technique, at normobaric and at hyperbaric pressure (10.1 MPa). 
      Statistical analysis revealed that high pressure increased NR1-1a / NR2A current 
      amplitudes. By contrast, high pressure decreased NR1-1b / NR2A current 
      amplitudes. These preliminary results demonstrate a differential high pressure 
      effect on two types of NMDAR subunits. Moreover, augmentation of the NR1-1a / 
      NR2A composition further supports the recently reported increase of NMDAR 
      synaptic response in rat hippocampus brain slices. These results support the 
      notion that increased NMDAR response, in addition to other mechanisms, plays an 
      important role in HPNS.
FAU - Mor, Amir
AU  - Mor A
AD  - PhD student in the Department of Physiology, Faculty of Health Sciences, 
      Ben-Gurion University of the Negev, Beer-Sheva, 8 Hameorer Street, Givataym 
      53220, Israel, E-mail: morami12@gmail.com.
FAU - Levy, Shiri
AU  - Levy S
FAU - Hollmann, Michael
AU  - Hollmann M
FAU - Gerossman, Yoram
AU  - Gerossman Y
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Diving Hyperb Med
JT  - Diving and hyperbaric medicine
JID - 101282742
EDAT- 2008/12/01 00:00
MHDA- 2008/12/01 00:01
CRDT- 2012/06/14 06:00
PHST- 2008/09/05 00:00 [received]
PHST- 2008/11/12 00:00 [accepted]
PHST- 2012/06/14 06:00 [entrez]
PHST- 2008/12/01 00:00 [pubmed]
PHST- 2008/12/01 00:01 [medline]
PST - ppublish
SO  - Diving Hyperb Med. 2008 Dec;38(4):194-6.