PMID- 22692990
OWN - NLM
STAT- MEDLINE
DCOM- 20121029
LR  - 20120704
IS  - 1439-7633 (Electronic)
IS  - 1439-4227 (Linking)
VI  - 13
IP  - 10
DP  - 2012 Jul 9
TI  - Cardiosulfa induces heart deformation in zebrafish through the AhR-mediated, 
      CYP1A-independent pathway.
PG  - 1483-9
LID - 10.1002/cbic.201200177 [doi]
AB  - Heart development is a complicated and elaborate biological process. To study 
      this and similar complicated process and diseases, the discovery and use of small 
      molecules for probing biological events is invaluable. As part of such an 
      investigation, we have identified cardiosulfa, a small molecule that induces 
      severely impaired heart morphology and function in zebrafish. The results of the 
      present study show that cardiosulfa-promoted heart deformation is protected by 
      negative regulators of the aryl hydrocarbon receptor (AhR) signaling pathway, 
      such as the AhR antagonist CH-223191 and an AhR2-morpholino antisense 
      oligonucleotide, zfahr2-MO. However, the toxic effect of cardiosulfa is not 
      alleviated by zfcyp1a-MO, a morpholino antisense oligo for cytochrome P450 1A 
      (CYP1A), which is the most well-characterized gene of the AhR pathway. Similar 
      results were obtained for the known AhR agonist PCB126. These observations 
      suggest that cardiosulfa causes heart deformation in zebrafish through the 
      AhR-mediated, CYP1A-independent pathway. Our results indicate that cardiosulfa 
      has potential as a novel type of a biological probe to investigate the AhR 
      pathway.
CI  - Copyright (c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Ko, Sung-Kyun
AU  - Ko SK
AD  - Center for Biofunctional Molecules, Department of Chemistry, Yonsei University, 
      120-749 Seoul, Korea.
FAU - Shin, Injae
AU  - Shin I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120612
PL  - Germany
TA  - Chembiochem
JT  - Chembiochem : a European journal of chemical biology
JID - 100937360
RN  - 0 (2-methyl-2H-pyrazole-3-carboxylic acid (2-methyl-4-o-tolylazophenyl)amide)
RN  - 0 (Azo Compounds)
RN  - 0 (Carbazoles)
RN  - 0 (Pyrazoles)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 0 (Sulfonamides)
RN  - 0 (Zebrafish Proteins)
RN  - 0 (cardiosulfa)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - Azo Compounds/chemistry/pharmacology
MH  - Carbazoles/chemistry/*pharmacology
MH  - Cytochrome P-450 CYP1A1/metabolism
MH  - Embryo, Nonmammalian/drug effects/metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Heart/*drug effects
MH  - Hep G2 Cells
MH  - Humans
MH  - Pyrazoles/chemistry/pharmacology
MH  - RNA Interference
MH  - RNA, Small Interfering/metabolism
MH  - Receptors, Aryl Hydrocarbon/antagonists & inhibitors/genetics/*metabolism
MH  - Signal Transduction
MH  - Sulfonamides/chemistry/*pharmacology
MH  - Transfection
MH  - Zebrafish/metabolism
MH  - Zebrafish Proteins/antagonists & inhibitors/*metabolism
EDAT- 2012/06/14 06:00
MHDA- 2012/10/30 06:00
CRDT- 2012/06/14 06:00
PHST- 2012/03/13 00:00 [received]
PHST- 2012/06/14 06:00 [entrez]
PHST- 2012/06/14 06:00 [pubmed]
PHST- 2012/10/30 06:00 [medline]
AID - 10.1002/cbic.201200177 [doi]
PST - ppublish
SO  - Chembiochem. 2012 Jul 9;13(10):1483-9. doi: 10.1002/cbic.201200177. Epub 2012 Jun 
      12.