PMID- 22693605
OWN - NLM
STAT- MEDLINE
DCOM- 20121022
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 5
DP  - 2012
TI  - Protoplasmic astrocytes enhance the ability of neural stem cells to differentiate 
      into neurons in vitro.
PG  - e38243
LID - 10.1371/journal.pone.0038243 [doi]
LID - e38243
AB  - Protoplasmic astrocytes have been reported to exhibit neuroprotective effects on 
      neurons, but there has been no direct evidence for a functional relationship 
      between protoplasmic astrocytes and neural stem cells (NSCs). In this study, we 
      examined neuronal differentiation of NSCs induced by protoplasmic astrocytes in a 
      co-culture model. Protoplasmic astrocytes were isolated from new-born and NSCs 
      from the E13-15 cortex of rats respectively. The differentiated cells labeled 
      with neuron-specific marker beta-tubulin III, were dramatically increased at 7 days 
      in the co-culture condition. Blocking the effects of brain-derived neurotrophic 
      factor (BDNF) with an anti-BDNF antibody reduced the number of neurons 
      differentiated from NSCs when co-cultured with protoplasmic astrocytes. In fact, 
      the content of BDNF in the supernatant obtained from protoplasmic astrocytes and 
      NSCs co-culture media was significantly greater than that from control media 
      conditions. These results indicate that protoplasmic astrocytes promote neuronal 
      differentiation of NSCs, which is driven, at least in part, by BDNF.
FAU - Liu, Yuan
AU  - Liu Y
AD  - The 3rd Department of Research Institute of Surgery, Daping Hospital, The Third 
      Military Medical University, State Key Laboratory of Trauma, Burns and Combined 
      Injury, Chongqing, People's Republic of China.
FAU - Wang, Li
AU  - Wang L
FAU - Long, Zaiyun
AU  - Long Z
FAU - Zeng, Lin
AU  - Zeng L
FAU - Wu, Yamin
AU  - Wu Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120531
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Astrocytes/*cytology
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - *Cell Differentiation
MH  - Cell Proliferation
MH  - Coculture Techniques
MH  - Cytoplasm/*metabolism
MH  - Neural Stem Cells/*cytology/metabolism
MH  - Neurons/*cytology
MH  - Rats
PMC - PMC3365019
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/06/14 06:00
MHDA- 2012/10/23 06:00
PMCR- 2012/05/31
CRDT- 2012/06/14 06:00
PHST- 2012/01/16 00:00 [received]
PHST- 2012/05/02 00:00 [accepted]
PHST- 2012/06/14 06:00 [entrez]
PHST- 2012/06/14 06:00 [pubmed]
PHST- 2012/10/23 06:00 [medline]
PHST- 2012/05/31 00:00 [pmc-release]
AID - PONE-D-12-01767 [pii]
AID - 10.1371/journal.pone.0038243 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(5):e38243. doi: 10.1371/journal.pone.0038243. Epub 2012 May 31.