PMID- 22695180
OWN - NLM
STAT- MEDLINE
DCOM- 20121101
LR  - 20211021
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 9
DP  - 2012 Jun 13
TI  - Prediction and identification of mouse cytotoxic T lymphocyte epitopes in Ebola 
      virus glycoproteins.
PG  - 111
LID - 10.1186/1743-422X-9-111 [doi]
AB  - BACKGROUND: Ebola viruses (EBOVs) cause severe hemorrhagic fever with a high 
      mortality rate. At present, there are no licensed vaccines or efficient therapies 
      to combat EBOV infection. Previous studies have shown that both humoral and 
      cellular immune responses are crucial for controlling Ebola infection. CD8+ T 
      cells play an important role in mediating vaccine-induced protective immunity. 
      The objective of this study was to identify H-2d-specific T cell epitopes in EBOV 
      glycoproteins (GPs). RESULTS: Computer-assisted algorithms were used to predict 
      H-2d-specific T cell epitopes in two species of EBOV (Sudan and Zaire) GP. The 
      predicted peptides were synthesized and identified in BALB/c mice immunized with 
      replication-deficient adenovirus vectors expressing the EBOV GP. Enzyme-linked 
      immunospot assays and intracellular cytokine staining showed that the peptides 
      RPHTPQFLF (Sudan EBOV), GPCAGDFAF and LYDRLASTV (Zaire EBOV) could stimulate 
      splenoctyes in immunized mice to produce large amounts of interferon-gamma. 
      CONCLUSION: Three peptides within the GPs of two EBOV strains were identified as 
      T cell epitopes. The identification of these epitopes should facilitate the 
      evaluation of vaccines based on the Ebola virus glycoprotein in a BALB/c mouse 
      model.
FAU - Wu, Shipo
AU  - Wu S
AD  - State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of 
      Microbiology and Epidemiology, No.20 Dongdajie Street, Fengtai district, Beijing 
      100071, People's Republic of China.
FAU - Yu, Ting
AU  - Yu T
FAU - Song, Xiaohong
AU  - Song X
FAU - Yi, Shaoqiong
AU  - Yi S
FAU - Hou, Lihua
AU  - Hou L
FAU - Chen, Wei
AU  - Chen W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120613
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (Ebola Vaccines)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Glycoproteins)
RN  - 0 (Vaccines, Synthetic)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Computational Biology
MH  - Ebola Vaccines/administration & dosage/immunology
MH  - Ebolavirus/*immunology
MH  - Enzyme-Linked Immunospot Assay
MH  - Epitopes, T-Lymphocyte/*immunology
MH  - Female
MH  - Genetic Vectors
MH  - Glycoproteins/*immunology
MH  - Interferon-gamma/metabolism
MH  - Leukocytes, Mononuclear/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Vaccines, Synthetic/administration & dosage/immunology
PMC - PMC3411508
EDAT- 2012/06/15 06:00
MHDA- 2012/11/02 06:00
PMCR- 2012/06/13
CRDT- 2012/06/15 06:00
PHST- 2011/07/26 00:00 [received]
PHST- 2012/06/13 00:00 [accepted]
PHST- 2012/06/15 06:00 [entrez]
PHST- 2012/06/15 06:00 [pubmed]
PHST- 2012/11/02 06:00 [medline]
PHST- 2012/06/13 00:00 [pmc-release]
AID - 1743-422X-9-111 [pii]
AID - 10.1186/1743-422X-9-111 [doi]
PST - epublish
SO  - Virol J. 2012 Jun 13;9:111. doi: 10.1186/1743-422X-9-111.