PMID- 22697426
OWN - NLM
STAT- MEDLINE
DCOM- 20130416
LR  - 20211021
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Print)
IS  - 1937-3341 (Linking)
VI  - 18
IP  - 21-22
DP  - 2012 Nov
TI  - Fast-degradable microbeads encapsulating human umbilical cord stem cells in 
      alginate for muscle tissue engineering.
PG  - 2303-14
LID - 10.1089/ten.TEA.2011.0658 [doi]
AB  - Human umbilical cord mesenchymal stem cells (hUCMSCs) are inexhaustible and can 
      be obtained without an invasive surgery. To date, there has been no report on 
      seeding hUCMSCs in three-dimensional scaffolds for muscle tissue engineering. The 
      objectives of this study were to (1) investigate hUCMSC seeding in a scaffold for 
      muscle engineering and (2) develop a novel construct consisting of 
      hUCMSC-encapsulating and fast-degradable microbeads inside a hydrogel matrix. The 
      rationale was that the hydrogel matrix would maintain the defect volume, while 
      the microbeads would degrade to release the cells and concomitantly create 
      macropores in the matrix. hUCMSCs were encapsulated in alginate-fibrin 
      microbeads, which were packed in an Arg-Gly-Asp (RGD)-modified alginate matrix 
      (AM). This construct is referred to as hUCMSC-microbead-AM. The control consisted 
      of the usual cell encapsulation in AM without microbeads (referred to as 
      hUCMSC-AM). In the hUCMSC-AM construct, the hUCMSCs showed as round dots with no 
      spreading at 1-14 days. In contrast, cells in the hUCMSC-microbead-AM construct 
      had a healthy spreading and elongated morphology. The microbeads successfully 
      degraded and released the cells at 8 days. Myogenic expressions for 
      hUCMSC-microbead-AM were more than threefold those of hUCMSC-AM (p<0.05). 
      Immunofluorescence for myogenic markers was much stronger for hUCMSC-microbead-AM 
      than hUCMSC-AM. Muscle creatine kinase of hUCMSC-microbead-AM at 14 days was 
      twofold that of hUCMSC-AM (p<0.05). In conclusion, hUCMSC encapsulation in novel 
      fast-degradable microbeads inside a hydrogel matrix was investigated for muscle 
      engineering. Compared to the usual method of seeding cells in a hydrogel matrix, 
      hUCMSC-microbead-AM construct had greatly improved cell viability and myogenic 
      differentiation, and hence, is promising to enhance muscle regeneration.
FAU - Liu, Jun
AU  - Liu J
AD  - Biomaterials and Tissue Engineering Division, Department of Endodontics, 
      Prosthodontics and Operative Dentistry, University of Maryland Dental School, 
      Baltimore, Maryland 21201, USA.
FAU - Zhou, Hongzhi
AU  - Zhou H
FAU - Weir, Michael D
AU  - Weir MD
FAU - Xu, Hockin H K
AU  - Xu HH
FAU - Chen, Qianming
AU  - Chen Q
FAU - Trotman, Carroll Ann
AU  - Trotman CA
LA  - eng
GR  - R21 DE022625/DE/NIDCR NIH HHS/United States
GR  - R01 DE14190/DE/NIDCR NIH HHS/United States
GR  - R01 DE013814/DE/NIDCR NIH HHS/United States
GR  - R41 DE01974/DE/NIDCR NIH HHS/United States
GR  - R01 DE014190/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120719
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (Alginates)
RN  - 0 (Hexuronic Acids)
RN  - 0 (Oligopeptides)
RN  - 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate)
RN  - 78VO7F77PN (arginyl-glycyl-aspartic acid)
RN  - 8A5D83Q4RW (Glucuronic Acid)
SB  - IM
MH  - Alginates/*pharmacology
MH  - Biodegradation, Environmental
MH  - Cell Survival/drug effects
MH  - Cells, Immobilized/cytology/drug effects/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Glucuronic Acid/pharmacology
MH  - Hexuronic Acids/pharmacology
MH  - Humans
MH  - Hydrogel, Polyethylene Glycol Dimethacrylate/chemistry
MH  - Mesenchymal Stem Cells/cytology/drug effects/metabolism
MH  - Microscopy, Electron, Scanning
MH  - *Microspheres
MH  - Muscle Development/drug effects/genetics
MH  - Muscles/drug effects/*physiology
MH  - Oligopeptides/pharmacology
MH  - Staining and Labeling
MH  - Stem Cells/*cytology/drug effects/metabolism
MH  - Tissue Engineering/*methods
MH  - Umbilical Cord/*cytology
PMC - PMC3482877
EDAT- 2012/06/16 06:00
MHDA- 2013/04/17 06:00
PMCR- 2013/11/01
CRDT- 2012/06/16 06:00
PHST- 2012/06/16 06:00 [entrez]
PHST- 2012/06/16 06:00 [pubmed]
PHST- 2013/04/17 06:00 [medline]
PHST- 2013/11/01 00:00 [pmc-release]
AID - 10.1089/ten.tea.2011.0658 [pii]
AID - 10.1089/ten.TEA.2011.0658 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2012 Nov;18(21-22):2303-14. doi: 10.1089/ten.TEA.2011.0658. 
      Epub 2012 Jul 19.