PMID- 22699773
OWN - NLM
STAT- MEDLINE
DCOM- 20121102
LR  - 20161125
IS  - 1464-0333 (Electronic)
IS  - 1464-0325 (Linking)
VI  - 14
IP  - 8
DP  - 2012 Aug
TI  - Changes in thyroid peroxidase activity in response to various chemicals.
PG  - 2121-6
LID - 10.1039/c2em30106g [doi]
AB  - Thyroperoxidase (TPO) is a large heme-containing glycoprotein that catalyzes the 
      transfer of iodine to thyroglobulin during thyroid hormone (TH) synthesis. 
      Previously, we established an in vitro assay for TPO activity based on human 
      recombinant TPO (hrTPO) stably transfected into human follicular thyroid 
      carcinoma (FTC-238) cells. It is important to determine whether environmental 
      chemicals can disrupt TPO activity because it is an important factor in the TH 
      axis. In this study, we used our assay to examine the changes in TPO activity in 
      response to various chemicals, including benzophenones (BPs), polycyclic aromatic 
      hydrocarbons (PAHs), and persistent organic pollutants (POPs). Overall, BPs, 
      PAHs, and POPs slightly altered TPO activity at low doses, as compared with the 
      positive controls methimazole (MMI), genistein, and 2,2',4,4'-tetrahydroxy BP. 
      Benzophenone, benzhydrol, 3-methylchloranthracene, pyrene, benzo(k)fluoranthene, 
      benzo(e)pyrene, perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), 
      and heptachlor decreased TPO activity, while 2,4-dihydroxy BP, 
      2,2'-dihydroxy-4-methoxy BP, and dibenzo(a,h)anthracene increased TPO activity. 
      From these data, we can predict the disruption of TPO activity by various 
      chemicals as a sensitive TH end point. TPO activity should be considered when 
      enacting measures to regulate environmental exposure to thyroid-disrupting 
      chemicals.
FAU - Song, Mee
AU  - Song M
AD  - Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and 
      Technology (KIST), P.O. Box 131, Cheongryang, Seoul, Korea.
FAU - Kim, Youn-Jung
AU  - Kim YJ
FAU - Park, Yong-Keun
AU  - Park YK
FAU - Ryu, Jae-Chun
AU  - Ryu JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120615
PL  - England
TA  - J Environ Monit
JT  - Journal of environmental monitoring : JEM
JID - 100968688
RN  - 0 (Endocrine Disruptors)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Fluorocarbons)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (Thyroid Hormones)
RN  - 554Z48XN5E (Methimazole)
RN  - 9010-34-8 (Thyroglobulin)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Cell Line
MH  - Endocrine Disruptors/toxicity
MH  - Environmental Pollutants/*toxicity
MH  - Fluorocarbons/toxicity
MH  - Humans
MH  - Methimazole/metabolism
MH  - Peroxidase/*metabolism
MH  - Polycyclic Aromatic Hydrocarbons/toxicity
MH  - Risk Assessment
MH  - Thyroglobulin/metabolism
MH  - Thyroid Gland/drug effects/*metabolism
MH  - Thyroid Hormones/metabolism
EDAT- 2012/06/16 06:00
MHDA- 2012/11/03 06:00
CRDT- 2012/06/16 06:00
PHST- 2012/06/16 06:00 [entrez]
PHST- 2012/06/16 06:00 [pubmed]
PHST- 2012/11/03 06:00 [medline]
AID - 10.1039/c2em30106g [doi]
PST - ppublish
SO  - J Environ Monit. 2012 Aug;14(8):2121-6. doi: 10.1039/c2em30106g. Epub 2012 Jun 
      15.