PMID- 22700284
OWN - NLM
STAT- MEDLINE
DCOM- 20130701
LR  - 20220410
IS  - 1573-7438 (Electronic)
IS  - 0049-4747 (Linking)
VI  - 45
IP  - 1
DP  - 2013 Jan
TI  - Effect of addition of coconut water (Cocos nucifera) to the freezing media on 
      post-thaw viability of boar sperm.
PG  - 101-6
LID - 10.1007/s11250-012-0179-0 [doi]
AB  - The aims of this experiment were to evaluate the addition of coconut water in 
      natura to the freezing media, compare the effect of deionized water vs filtered 
      water of coconut over the post-thaw seminal characteristics, and evaluate the 
      effect of the deionized water and in natura coconut water on the seminal 
      characteristics of boar sperm at different post-thaw times. Thirty-four 
      ejaculates were used divided in three aliquots which received one of the 
      following treatments (T): T1, LEY (bidistilled water, lactose, and egg yolk) and 
      LEYGO (LEY + glycerol and Orvus ET paste); T2, LEY(A) (coconut deionized water, 
      lactose, and egg yolk)-LEYGO(A); and T3, LEY(B) (in natura coconut water, 
      lactose, and egg yolk)-LEYGO(B). Samples of boar semen were frozen according to 
      the Westendorf method, thawed at 38 degrees C, and evaluated at three incubation times 
      (0, 30, and 60 min). Seminal characteristics assessed were motility (Mot), 
      acrosomal integrity (AInt), membrane integrity (MInt), and mitochondrial activity 
      (MAct). T1 showed a higher percentage of viable sperm than T3 (Mot 36.5 vs 5.4 %, 
      AInt 61.8 vs 41.2 %, MInt 50.4 vs 41.3 %, and MAct 56.9 vs 50.5 %). T2 kept a 
      higher percentage of viable sperm at all incubation times. In natura coconut 
      water showed a detrimental effect over the viability of the frozen-thawed boar 
      semen. Deionized coconut water improved the boar semen viability post-thaw, 
      outperforming results of in natura coconut water.
FAU - Bottini-Luzardo, Maria
AU  - Bottini-Luzardo M
AD  - Facultad de Medicina Veterinaria y Zootecnia, Universidad Autonoma de Yucatan, km 
      15.5 Carretera Merida-Xmatkuil, CP 97100, Merida, Yucatan, Mexico.
FAU - Centurion-Castro, Fernando
AU  - Centurion-Castro F
FAU - Alfaro-Gamboa, Militza
AU  - Alfaro-Gamboa M
FAU - Ake-Lopez, Ricardo
AU  - Ake-Lopez R
FAU - Herrera-Camacho, Jose
AU  - Herrera-Camacho J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120615
PL  - United States
TA  - Trop Anim Health Prod
JT  - Tropical animal health and production
JID - 1277355
RN  - 0 (Cryoprotective Agents)
RN  - 0 (Culture Media)
RN  - J2B2A4N98G (Lactose)
SB  - IM
MH  - Acrosome/drug effects
MH  - Analysis of Variance
MH  - Animals
MH  - Cocos/*chemistry
MH  - Cryopreservation/methods/*veterinary
MH  - Cryoprotective Agents/analysis/*pharmacology
MH  - Culture Media/analysis/*pharmacology
MH  - Egg Yolk
MH  - Lactose
MH  - Male
MH  - Mitochondria/drug effects
MH  - Semen Preservation/methods/*veterinary
MH  - Sperm Motility/drug effects
MH  - Spermatozoa/cytology/*drug effects
MH  - *Sus scrofa
EDAT- 2012/06/16 06:00
MHDA- 2013/07/03 06:00
CRDT- 2012/06/16 06:00
PHST- 2012/05/09 00:00 [accepted]
PHST- 2012/06/16 06:00 [entrez]
PHST- 2012/06/16 06:00 [pubmed]
PHST- 2013/07/03 06:00 [medline]
AID - 10.1007/s11250-012-0179-0 [doi]
PST - ppublish
SO  - Trop Anim Health Prod. 2013 Jan;45(1):101-6. doi: 10.1007/s11250-012-0179-0. Epub 
      2012 Jun 15.