PMID- 2270083
OWN - NLM
STAT- MEDLINE
DCOM- 19910220
LR  - 20190725
IS  - 0026-8925 (Print)
IS  - 0026-8925 (Linking)
VI  - 223
IP  - 3
DP  - 1990 Sep
TI  - Mutant of the glutamine transfer RNA gene as UGA suppressor in Escherichia coli.
PG  - 433-7
AB  - A UGA suppressor derived from a glutamine tRNA gene of Escherichia coli K12 was 
      isolated and characterized. Phages carrying the suppressor su+2UGA could be 
      obtained only from a hybrid transducing phage, h80cI857psu+2oc, but not from the 
      original transducing phage lambda cI857psu+2oc. By DNA sequence analysis, it was 
      found that the su+2 UGA suppressor obtained has two mutations; one is in the 
      anticodon (TTA----TCA), as expected, and the other (C----T) is at the 7th 
      position from the 3' end of tRNA(2Gln). The significance of these mutations and 
      the lethal effect on phage lambda of the increased amounts of UGA suppressor 
      tRNAs are discussed.
FAU - Inokuchi, H
AU  - Inokuchi H
AD  - Department of Biophysics, Faculty of Science, Kyoto University, Japan.
FAU - Kondo, K
AU  - Kondo K
FAU - Yoshimura, M
AU  - Yoshimura M
FAU - Ozeki, H
AU  - Ozeki H
LA  - eng
SI  - GENBANK/X55374
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Mol Gen Genet
JT  - Molecular & general genetics : MGG
JID - 0125036
RN  - 0 (RNA, Transfer, Gln)
SB  - IM
MH  - Bacteriophages/genetics
MH  - Base Sequence
MH  - Escherichia coli/*genetics
MH  - *Genes, Suppressor
MH  - Genetic Complementation Test
MH  - Molecular Sequence Data
MH  - *Mutation
MH  - Nucleic Acid Conformation
MH  - RNA, Transfer, Gln/*genetics
MH  - Transduction, Genetic
EDAT- 1990/09/01 00:00
MHDA- 1990/09/01 00:01
CRDT- 1990/09/01 00:00
PHST- 1990/09/01 00:00 [pubmed]
PHST- 1990/09/01 00:01 [medline]
PHST- 1990/09/01 00:00 [entrez]
AID - 10.1007/BF00264450 [doi]
PST - ppublish
SO  - Mol Gen Genet. 1990 Sep;223(3):433-7. doi: 10.1007/BF00264450.