PMID- 22700973
OWN - NLM
STAT- MEDLINE
DCOM- 20121105
LR  - 20211021
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 287
IP  - 33
DP  - 2012 Aug 10
TI  - The effects of low-dose ionizing radiation in the activated rat basophilic 
      leukemia (RBL-2H3) mast cells.
PG  - 27789-95
LID - 10.1074/jbc.M112.378497 [doi]
AB  - Mast cells play important roles in many biological responses, such as those 
      during allergic diseases and inflammatory disorders. Although laser and UV 
      irradiation have immunosuppressive effects on inflammatory diseases by 
      suppressing mast cells, little is known about the effects of gamma-ionizing radiation 
      on mast cells. In this study, we investigated the effects of gamma-ionizing radiation 
      on RBL-2H3 cells, a convenient model system for studying regulated secretion by 
      mast cells. Low-dose radiation (<0.1 gray (Gy)) did not induce cell death, but 
      high-dose radiation (>0.5 Gy) induced apoptosis. Low-dose ionizing radiation 
      significantly suppressed the release of mediators (histamine, beta-hexosaminidase, 
      IL-4, and tumor necrosis factor-alpha) from immunoglobulin E (IgE)-sensitized RBL-2H3 
      cells. To determine the mechanism of mediator release inhibition by ionizing 
      radiation, we examined the activation of intracellular signaling molecules such 
      as Lyn, Syk, phospholipase Cgamma, PKCs, and MAPK, and intracellular free calcium 
      concentrations ([Ca(2+)](i)). The phosphorylation of signaling molecules 
      following stimulation of high-affinity IgE receptor I (FcepsilonRI) was specifically 
      inhibited by low-dose ionizing radiation (0.01 Gy). These results were due to the 
      suppression of FcepsilonRI expression by the low-dose ionizing radiation. Therefore, 
      low-dose ionizing radiation (0.01 Gy) may function as a novel inhibitor of mast 
      cell activation.
FAU - Joo, Hae Mi
AU  - Joo HM
AD  - Radiation Effect Research Team, Radiation Health Research Institute, Korea Hydro 
      and Nuclear Power Co., Ltd., Seoul 132-703, Korea.
FAU - Nam, Seon Young
AU  - Nam SY
FAU - Yang, Kwang Hee
AU  - Yang KH
FAU - Kim, Cha Soon
AU  - Kim CS
FAU - Jin, Young Woo
AU  - Jin YW
FAU - Kim, Ji Young
AU  - Kim JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120614
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Animals
MH  - Apoptosis/radiation effects
MH  - Cell Line, Tumor
MH  - Dose-Response Relationship, Radiation
MH  - *Gamma Rays
MH  - Leukemia, Basophilic, Acute/*metabolism/pathology
MH  - Mast Cells/*metabolism/pathology
MH  - Neoplasm Proteins/*metabolism
MH  - Phosphorylation/radiation effects
MH  - Rats
MH  - Signal Transduction/*radiation effects
PMC - PMC3431648
EDAT- 2012/06/16 06:00
MHDA- 2012/11/06 06:00
PMCR- 2013/08/10
CRDT- 2012/06/16 06:00
PHST- 2012/06/16 06:00 [entrez]
PHST- 2012/06/16 06:00 [pubmed]
PHST- 2012/11/06 06:00 [medline]
PHST- 2013/08/10 00:00 [pmc-release]
AID - S0021-9258(20)47801-9 [pii]
AID - M112.378497 [pii]
AID - 10.1074/jbc.M112.378497 [doi]
PST - ppublish
SO  - J Biol Chem. 2012 Aug 10;287(33):27789-95. doi: 10.1074/jbc.M112.378497. Epub 
      2012 Jun 14.