PMID- 22702058
OWN - NLM
STAT- MEDLINE
DCOM- 20130215
LR  - 20181201
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 26
IP  - 5
DP  - 2012 May
TI  - [Promotion effect of notochordal cells conditioned medium on proliferation and 
      differentiation of bone marrow mesenchymal stem cells].
PG  - 601-6
AB  - OBJECTIVE: To investigate the effect of notochordal cells (NCs) conditioned 
      medium (NCCM) on the proliferation and differentiation of bone marrow mesenchymal 
      stem cells (BMSCs). METHODS: NCs and BMSCs were isolated from the thoracolumbar 
      intervertebral disc and the femurs of 4-week-old Japanese white rabbits, 
      respectively. NCs were cultured with DMEM/F12 medium containing 15% FBS for 5 
      days to prepare NCCM. The experiment consisted of 2 groups: BMSCs were cultured 
      with NCCM in experimental group and with DMEM/F12 medium containing 15% FBS in 
      control group. The proliferation of BMSCs was assessed by cell counting kit-8 at 
      1, 3, 5, 7, 9, and 14 days after culture; the expression of proteoglycan and 
      collagen type II were determined by immunofluorescence and real-time fluorescent 
      quantitative PCR at 7 and 14 days after culture. RESULTS: NCs and BMSCs were 
      successfully isloated. At 5, 7, 9, and 14 days, the number of BMSCs in the 
      experimental group was significantly more than those in the control group (P < 
      0.05). At 7 and 14 days, there was no expression or less expression of 
      proteoglycan and collagen type II in the control group; however, there was a lot 
      of expression of proteoglycan and collagen type II in the experimental group, and 
      the expressions were higher at 14 days than at 7 days. At 7 and 14 days after 
      culture, the mRNA expressions of proteoglycan and collagen type II were 
      significantly higher in the experimental group than in the control group (P < 
      0.05), and at 14 days than at 7 days in the experimental group (P < 0.05). 
      CONCLUSION: NCCM can promote the proliferation and the differentiation of BMSCs 
      into chondroyte-like cells, which provides the basis for NCs and BMSCs as seed 
      cells in the treatment of degenerative disc disease.
FAU - Ma, Kaige
AU  - Ma K
AD  - Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan Hubei 430022, PR China.
FAU - Shao, Zengwu
AU  - Shao Z
FAU - Wang, Baichuan
AU  - Wang B
FAU - Zhang, Yannan
AU  - Zhang Y
FAU - Yang, Shuhua
AU  - Yang S
FAU - Liu, Tao
AU  - Liu T
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Collagen Type II)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Proteoglycans)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Cell Culture Techniques/methods
MH  - *Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Collagen Type II/genetics/metabolism
MH  - Culture Media, Conditioned/metabolism/*pharmacology
MH  - Female
MH  - Intervertebral Disc/cytology
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Notochord/*cytology
MH  - Proteoglycans/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rabbits
MH  - Tissue Engineering/methods
EDAT- 2012/06/19 06:00
MHDA- 2013/02/16 06:00
CRDT- 2012/06/19 06:00
PHST- 2012/06/19 06:00 [entrez]
PHST- 2012/06/19 06:00 [pubmed]
PHST- 2013/02/16 06:00 [medline]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2012 May;26(5):601-6.