PMID- 2270536
OWN - NLM
STAT- MEDLINE
DCOM- 19910221
LR  - 20031114
IS  - 0340-6245 (Print)
IS  - 0340-6245 (Linking)
VI  - 64
IP  - 2
DP  - 1990 Oct 22
TI  - Potentiation of the antithrombotic action of dermatan sulphate by small amounts 
      of heparin.
PG  - 290-3
AB  - We have examined the effect in impairing thrombus formation of a preparation of 
      dermatan sulphate (DS) alone and DS plus small amounts of unfractionated heparin 
      (UFH). In rabbits given a dose of 150 micrograms/kg of DS alone, there was 
      minimal reduction in serum-induced stasis thrombosis (Wessler model), with an 
      overall score of 92.5% (100% = no impairment of thrombus formation). When the 
      same dose of DS containing UFH was given (two different subgroups given DS 
      containing 0.25 and 2.5% heparin by dry weight, respectively), the overall 
      thrombus score was reduced to 60% (P less than 0.003), with no significant 
      difference between the two subgroups. To achieve a comparable result with DS 
      alone, a dose of 1 mg/kg was required. We conclude that very small amounts of UFH 
      significantly enhance the antithrombotic action of DS, by a mechanism that has 
      yet to be determined.
FAU - Thomas, D P
AU  - Thomas DP
AD  - Division of Haematology, National Institute for Biological Standards and Control, 
      Potters Bar, Hertfordshire, U.K.
FAU - Gray, E
AU  - Gray E
FAU - Merton, R E
AU  - Merton RE
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Thromb Haemost
JT  - Thrombosis and haemostasis
JID - 7608063
RN  - 24967-94-0 (Dermatan Sulfate)
RN  - 9005-49-6 (Heparin)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Animals
MH  - Blood Coagulation/drug effects
MH  - Dermatan Sulfate/*administration & dosage
MH  - Drug Evaluation, Preclinical
MH  - Drug Synergism
MH  - Heparin/*administration & dosage
MH  - Rabbits
MH  - Thrombin/biosynthesis
MH  - Thrombosis/*prevention & control
EDAT- 1990/10/22 00:00
MHDA- 1990/10/22 00:01
CRDT- 1990/10/22 00:00
PHST- 1990/10/22 00:00 [pubmed]
PHST- 1990/10/22 00:01 [medline]
PHST- 1990/10/22 00:00 [entrez]
PST - ppublish
SO  - Thromb Haemost. 1990 Oct 22;64(2):290-3.