PMID- 22708121
OWN - NLM
STAT- MEDLINE
DCOM- 20120709
LR  - 20190612
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 421
IP  - 2
DP  - 2012 May 4
TI  - Juglans mandshurica leaf extract protects skin fibroblasts from damage by 
      regulating the oxidative defense system.
PG  - 343-8
AB  - Skin is mainly damaged by genetic and environmental factors such as ultraviolet 
      light, xenobiotics, hormonal changes, heat, and smoking. ROS production is 
      commonly involved in the pathogenesis of skin damage induced by these factors, 
      causing skin aging, including wrinkling, by activating the metalloproteinases 
      (MMP-1) that break down type I collagen (COL1A1). The walnut tree Juglans 
      mandshurica MAX. (JM) is found in China, Siberia and Korea. JM has been reported 
      to have various pharmacological activities, such as anti-tumor, anti-oxidative, 
      and anti-bacterial effects. In the present study, we investigated the protective 
      effect of JM leaf extract (JME) against oxidative stress in HS68 human skin 
      fibroblasts. JME significantly and dose-dependently protected HS68 cells against 
      H(2)O(2)-induced damage, as assessed by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate 
      dehydrogenase assay. Other assays demonstrated that JME protected HS68 cells by 
      regulating ROS production and increasing levels of glutathione, heme oxygenase-1, 
      and activated NF-E2-related factor 2. JME additionally prevented the elevation of 
      MMP-1 and reduction of COL1A1 induced by H(2)O(2). It also inhibited H(2)O(2)-induced 
      phosphorylation of ERK, p38, and JNK. These results indicate that JME protects 
      human skin fibroblasts from H(2)O(2)-induced damage by regulating the oxidative 
      defense system.
FAU - Park, Gunhyuk
AU  - Park G
AD  - Department of Life and Nanopharmaceutical Science, and Kyung Hee East-West 
      Pharmaceutical Research Institute, Kyung Hee University, 1 Hoegi-dong, 
      Dongdaemun-gu, Seoul 130-701, Republic of Korea.
FAU - Jang, Dae Sik
AU  - Jang DS
FAU - Oh, Myung Sook
AU  - Oh MS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120409
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.14.14.18 (HMOX1 protein, human)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Cell Line
MH  - *Cytoprotection
MH  - Fibroblasts/drug effects
MH  - Glutathione/metabolism
MH  - Heme Oxygenase-1/metabolism
MH  - Humans
MH  - Hydrogen Peroxide/pharmacology
MH  - Juglans/*chemistry
MH  - Oxidative Stress/*drug effects
MH  - Plant Leaves/*chemistry
MH  - Skin/cytology/*drug effects
EDAT- 2012/06/19 06:00
MHDA- 2012/07/10 06:00
CRDT- 2012/06/19 06:00
PHST- 2012/06/19 06:00 [entrez]
PHST- 2012/06/19 06:00 [pubmed]
PHST- 2012/07/10 06:00 [medline]
AID - S0006-291X(12)00649-3 [pii]
AID - 10.1016/j.bbrc.2012.04.013 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2012 May 4;421(2):343-8. doi: 
      10.1016/j.bbrc.2012.04.013. Epub 2012 Apr 9.