PMID- 22708664
OWN - NLM
STAT- MEDLINE
DCOM- 20130429
LR  - 20120727
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 80
IP  - 2
DP  - 2012 Aug
TI  - Assessment of copy-number variation in the NKG2C receptor gene in a single-tube 
      and characterization of a reference cell panel, using standard polymerase chain 
      reaction.
PG  - 184-7
LID - 10.1111/j.1399-0039.2012.01911.x [doi]
AB  - Natural killer (NK) and T-lymphocytes monitor human leukocyte antigen (HLA)-E 
      expression through CD94:NKG2 heterodimers. Structural polymorphism is not a 
      hallmark for NK-complex genes on chromosome 12, except for complete NKG2C 
      deletion in some humans. We present a method for fast, simple and accurate 
      assessment of NKG2C copy-number variation - presence or absence in the genome of 
      an NKG2C gene, in homo- or heterozygosis, is detected by a single conventional 
      polymerase chain reaction that yields amplicons of different lengths in each 
      genotype. We have also determined the NKG2C genotypes of a reference cell panel 
      comprising 13 NK- and tumour-cell lines and 39 Epstein-Barr virus transformed 
      cells from the International Histocompatibility Workshop. Our results should 
      facilitate research on the importance of NKG2C and its deletion for immunity.
CI  - (c) 2012 John Wiley & Sons A/S.
FAU - Moraru, M
AU  - Moraru M
AD  - Laboratorio de Inmunogenetica-HLA, Servicio de Inmunologia, Hospital 
      Universitario Puerta de Hierro, Majadahonda, Madrid, Spain.
FAU - Canizares, M
AU  - Canizares M
FAU - Muntasell, A
AU  - Muntasell A
FAU - de Pablo, R
AU  - de Pablo R
FAU - Lopez-Botet, M
AU  - Lopez-Botet M
FAU - Vilches, C
AU  - Vilches C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120618
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (DNA Primers)
RN  - 0 (KLRC2 protein, human)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily C)
SB  - IM
MH  - Cell Line, Transformed
MH  - Cell Line, Tumor
MH  - Chromosomes, Human, Pair 12/genetics
MH  - DNA Copy Number Variations/*genetics
MH  - DNA Primers
MH  - Gene Dosage
MH  - Herpesvirus 4, Human/genetics
MH  - Heterozygote
MH  - Homozygote
MH  - Humans
MH  - Killer Cells, Natural/cytology/immunology/*metabolism
MH  - NK Cell Lectin-Like Receptor Subfamily C/*genetics/immunology
MH  - Polymerase Chain Reaction/*methods
MH  - T-Lymphocytes/cytology/immunology/*metabolism
EDAT- 2012/06/20 06:00
MHDA- 2013/04/30 06:00
CRDT- 2012/06/20 06:00
PHST- 2012/02/20 00:00 [received]
PHST- 2012/04/13 00:00 [revised]
PHST- 2012/05/17 00:00 [accepted]
PHST- 2012/06/20 06:00 [entrez]
PHST- 2012/06/20 06:00 [pubmed]
PHST- 2013/04/30 06:00 [medline]
AID - 10.1111/j.1399-0039.2012.01911.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2012 Aug;80(2):184-7. doi: 10.1111/j.1399-0039.2012.01911.x. 
      Epub 2012 Jun 18.