PMID- 22709934
OWN - NLM
STAT- MEDLINE
DCOM- 20121210
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 38
IP  - 1
DP  - 2012 Oct-Dec
TI  - A universal amplified strategy for aptasensors: enhancing sensitivity through 
      allostery-triggered enzymatic recycling amplification.
PG  - 121-5
LID - 10.1016/j.bios.2012.05.008 [doi]
AB  - A universal amplified sensing strategy based on endonuclease was developed for 
      designing fluorescence aptasensors. By employing hairpin-structured design for 
      both recognition and reporter probes to decrease background signal, and a nicking 
      endonuclease to perform target-triggered enzymatic recycling amplification, the 
      proposed biosensor showed high sensitivity to target protein. To demonstrate the 
      feasibility of the design, immunoglobulin E (IgE) was studied as a model target. 
      Upon the addition of target protein, the specific formation of IgE/aptamer 
      complex induced the releasing of the 37-mer fragment which partially hybridized 
      with the molecular beacon (MB) probe. In the presence of endonuclease Nt.BbvCI, 
      the MB was cleaved into two parts. Then, the released 37-mer fragment hybridized 
      with another MB, and triggered the second cycle of cleavage, leading to an 
      accumulation of fluorescence signals. Under the optimal conditions, a detection 
      limit of 5 pM was obtained. The proposed sensing system was used for detection of 
      IgE in complex biological samples with satisfactory results.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Feng, Kejun
AU  - Feng K
AD  - Shandong Provincial Key Laboratory of Life-organic Analysis, College of Chemistry 
      and Chemical Engineering, Qufu Normal University, Qufu Shandong 273165, China.
FAU - Kong, Rongmei
AU  - Kong R
FAU - Wang, Hua
AU  - Wang H
FAU - Zhang, Shufang
AU  - Zhang S
FAU - Qu, Fengli
AU  - Qu F
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120526
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Molecular Probes)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.1.- (Endonucleases)
SB  - IM
MH  - Allosteric Regulation
MH  - Aptamers, Nucleotide/*chemistry
MH  - Biosensing Techniques/*methods
MH  - Endonucleases/metabolism
MH  - Humans
MH  - Immunoglobulin E/analysis/*blood
MH  - Molecular Probes/chemistry/metabolism
MH  - Sensitivity and Specificity
MH  - Spectrometry, Fluorescence/methods
EDAT- 2012/06/20 06:00
MHDA- 2012/12/12 06:00
CRDT- 2012/06/20 06:00
PHST- 2012/03/21 00:00 [received]
PHST- 2012/05/06 00:00 [revised]
PHST- 2012/05/08 00:00 [accepted]
PHST- 2012/06/20 06:00 [entrez]
PHST- 2012/06/20 06:00 [pubmed]
PHST- 2012/12/12 06:00 [medline]
AID - S0956-5663(12)00288-6 [pii]
AID - 10.1016/j.bios.2012.05.008 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2012 Oct-Dec;38(1):121-5. doi: 10.1016/j.bios.2012.05.008. 
      Epub 2012 May 26.