PMID- 22713986 OWN - NLM STAT- MEDLINE DCOM- 20121017 LR - 20190923 IS - 1738-8872 (Electronic) IS - 1017-7825 (Linking) VI - 22 IP - 8 DP - 2012 Aug TI - Microbial dynamics of commercial makgeolli depending on the storage temperature. PG - 1101-6 AB - Market fresh makgeolli was stored at different temperatures of 4 degrees C and 25 degrees C to assess the change of the microbial diversity according to the storage temperature and period. Yeast counts increased until day 3 of storage and decreased thereafter. General and lactic acid bacterial counts continuously increased during storage. The data indicated that the control of growth of microorganisms, particularly general bacteria and lactic acid bacteria (LAB), is essential. Total acid levels started to decrease in the makgeolli stored at 4 degrees C, and increased from day 6 of storage in the makgeolli stored at 25 degrees C. The increase of total acid in the non-refrigerated condition greatly affected the quality of makgeolli. In both the fresh makgeolli samples stored at 4 degrees C and 25 degrees C, yeast (Saccharomyces cerevisiae) and molds (Aspergillus tubingensis, Candida glaebosa, and Aspergillus niger) were noted. Denaturing gradient gel electrophoresis (DGGE) band patterns were almost constant regardless of the storage period. As for bacteria, Lactobacillus crustorum, L. brevis, and Microlaena stipoides were found in the makgeolli stored at 4 degrees C, and L. crustorum, Lactobacillus sp., L. plantarum, L. brevis, L. rhamnosus, and L. similis were found in the makgeolli stored at 25 degrees C. In particular, in the makgeolli stored at 25 degrees C, L. crustorum and L. plantarum presented dark bands and were identified as the primary microorganisms that affected spoilage of fresh makgeolli. FAU - Kim, Hye-Ryun AU - Kim HR AD - Korean Alcoholic Beverage Research Center, Korea Food Research Institute, Seongnam-si 463-746, Korea. FAU - Lee, Ae Ran AU - Lee AR FAU - Kim, Jae-Ho AU - Kim JH FAU - Ahn, Byung-Hak AU - Ahn BH LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Korea (South) TA - J Microbiol Biotechnol JT - Journal of microbiology and biotechnology JID - 9431852 SB - IM MH - Bacteria/classification/genetics/*isolation & purification MH - Bacterial Load MH - *Biodiversity MH - Colony Count, Microbial MH - Denaturing Gradient Gel Electrophoresis MH - *Food Microbiology MH - Fungi/classification/genetics/*isolation & purification MH - Temperature EDAT- 2012/06/21 06:00 MHDA- 2012/10/18 06:00 CRDT- 2012/06/21 06:00 PHST- 2012/06/21 06:00 [entrez] PHST- 2012/06/21 06:00 [pubmed] PHST- 2012/10/18 06:00 [medline] AID - JMB022-08-09 [pii] AID - 10.4014/jmb.1112.12063 [doi] PST - ppublish SO - J Microbiol Biotechnol. 2012 Aug;22(8):1101-6. doi: 10.4014/jmb.1112.12063.