PMID- 22714916
OWN - NLM
STAT- MEDLINE
DCOM- 20121214
LR  - 20211021
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 39
IP  - 9
DP  - 2012 Sep
TI  - Silencing SOCS3 could inhibit TNF-alpha induced apoptosis in 3T3-L1 and mouse 
      preadipocytes.
PG  - 8853-60
LID - 10.1007/s11033-012-1749-y [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a pro-inflammatory cytokine involved in 
      the apoptosis of many types of cells. In this study we demonstrated the effect of 
      (suppressor of cytokine signalling-3) SOCS3 siRNA on TNF-alpha induced apoptosis in 
      3T3-L1 preadipocytes and mouse preadipocytes. 3T3-L1 preadipocytes and mouse 
      preadipocytes were transfected with SOCS3 siRNA, and then the cells were treated 
      with TNF-alpha at 100 ng/mL for 24 h. We used fluorescence microscope to observe 
      morphological changes during apoptosis after Hoechst 33258 and PI staining. 
      Quantitative PCR and Western blotting were used to measure the expression of 
      apoptosis-associated gene c-myc, survivin, mcl-1, bcl-2, bax, NF-kappaB, and the key 
      genes of the JAK/STAT3 pathway including SOCS1, SOCS2, JAK2, STAT3. Compared with 
      control group, the number of cells apoptosis was decreased remarkably in SOCS3 
      siRNA group (P < 0.01). The expression of apoptotic suppressor genes c-myc, 
      survivin, mcl-1, bcl-2 and NF-kappaB were up-regulated markedly (P < 0.01); in 
      contrast, apoptotic gene bax was down-regulated (P < 0.05). Western blotting 
      showed that the protein expressions of bcl-2 and NF-kappaB were increased remarkably 
      (P < 0.01), while the protein expression of bax was decreased remarkably (P < 
      0.05). The expression of the JAK/STAT3 pathway key gene SOCS1 mRNA was 
      down-regulated markedly (P < 0.05), but the key protein p-STAT3 was up-regulated 
      (P < 0.05). Taken together, our data established that silenced SOCS3 can regulate 
      the expression of apoptosis-associated genes via the JAK/STAT3 pathway, and 
      effectively inhibit TNF-alpha induced apoptosis in 3T3-L1 preadipocytes and mouse 
      preadipocytes.
FAU - Zhao, Xia
AU  - Zhao X
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling 
      712100, Shaanxi, China. zhaoxia16861@163.com
FAU - Qi, Renli
AU  - Qi R
FAU - Sun, Chao
AU  - Sun C
FAU - Xie, Yunfei
AU  - Xie Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120620
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (Socs3 protein, mouse)
RN  - 0 (Suppressor of Cytokine Signaling 3 Protein)
RN  - 0 (Suppressor of Cytokine Signaling Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.10.2 (Janus Kinase 2)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects/*genetics
MH  - Cell Line
MH  - Gene Expression Regulation
MH  - *Gene Silencing
MH  - Janus Kinase 2/genetics
MH  - Mice
MH  - RNA Interference
MH  - STAT3 Transcription Factor/genetics
MH  - Signal Transduction
MH  - Suppressor of Cytokine Signaling 3 Protein
MH  - Suppressor of Cytokine Signaling Proteins/*genetics
MH  - Transfection
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2012/06/21 06:00
MHDA- 2012/12/15 06:00
CRDT- 2012/06/21 06:00
PHST- 2012/01/05 00:00 [received]
PHST- 2012/06/07 00:00 [accepted]
PHST- 2012/06/21 06:00 [entrez]
PHST- 2012/06/21 06:00 [pubmed]
PHST- 2012/12/15 06:00 [medline]
AID - 10.1007/s11033-012-1749-y [doi]
PST - ppublish
SO  - Mol Biol Rep. 2012 Sep;39(9):8853-60. doi: 10.1007/s11033-012-1749-y. Epub 2012 
      Jun 20.