PMID- 22714954
OWN - NLM
STAT- MEDLINE
DCOM- 20130723
LR  - 20211021
IS  - 1476-5535 (Electronic)
IS  - 1367-5435 (Linking)
VI  - 39
IP  - 10
DP  - 2012 Oct
TI  - Optimization of fermentation parameters in phage production using response 
      surface methodology.
PG  - 1515-22
AB  - Previously, we used computer-controlled fermentation technology to improve the 
      yield of filamentous phage produced in Escherichia coli by 10-fold (Grieco et 
      al., Bioprocess Biosyst Eng 32:773-779, 2009). In the current study, three major 
      fermentation parameters (temperature, dissolved oxygen [DO], and pH) were 
      investigated using design of experiments (DOE) methodology. Response surface 
      methodology (RSM) was employed to create a process model and determine the 
      optimal conditions for maximal phage production. The experimental data fitted 
      best to a quadratic model (p < 0.0001). Temperature and pH, but not DO, proved to 
      be significant variables. The model predicted a theoretical optimal condition for 
      maximal bacteriophage production at temperature of 28.1  degrees C and pH 6.9. A 
      validation run resulted in phage production [3.49 x 10(11) transducing units 
      (TU)/mL] comparable to the predicted value (2.86 x 10(11) TU/mL). This 
      represented a 7-fold increase in phage production above that obtained without 
      optimization, resulting in a 70-fold increase above that achieved by shake flask 
      culture alone.
FAU - Grieco, Sung-Hye H
AU  - Grieco SH
AD  - Centre for Blood Research, University of British Columbia, Vancouver, BC V6T 1Z3, 
      Canada. sunghye.grieco@ubc.ca
FAU - Wong, Ann Y K
AU  - Wong AY
FAU - Dunbar, W Scott
AU  - Dunbar WS
FAU - MacGillivray, Ross T A
AU  - MacGillivray RT
FAU - Curtis, Susan B
AU  - Curtis SB
LA  - eng
GR  - Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120620
PL  - Germany
TA  - J Ind Microbiol Biotechnol
JT  - Journal of industrial microbiology & biotechnology
JID - 9705544
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Analysis of Variance
MH  - Escherichia coli/metabolism/*virology
MH  - *Fermentation
MH  - Hydrogen-Ion Concentration
MH  - Inovirus/*growth & development
MH  - Models, Biological
MH  - Oxygen/metabolism
MH  - Reproducibility of Results
MH  - Temperature
EDAT- 2012/06/21 06:00
MHDA- 2013/07/24 06:00
CRDT- 2012/06/21 06:00
PHST- 2012/01/24 00:00 [received]
PHST- 2012/05/04 00:00 [accepted]
PHST- 2012/06/21 06:00 [entrez]
PHST- 2012/06/21 06:00 [pubmed]
PHST- 2013/07/24 06:00 [medline]
AID - 10.1007/s10295-012-1148-3 [doi]
PST - ppublish
SO  - J Ind Microbiol Biotechnol. 2012 Oct;39(10):1515-22. doi: 
      10.1007/s10295-012-1148-3. Epub 2012 Jun 20.