PMID- 22715695
OWN - NLM
STAT- MEDLINE
DCOM- 20140730
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 3
DP  - 2012 Mar
TI  - [Determination of 10 heterocyclic aromatic amines in beef jerky by high 
      performance liquid chromatography].
PG  - 285-91
AB  - An analytical method was developed for the simultaneous determination of 10 
      heterocyclic aromatic amines (HAAs) in beef jerky by solid phase extraction-high 
      performance liquid chromatography (SPE-HPLC). The HAAs were eluted from an 
      Extrelut NT 20 SPE column with 60 mL dichlormethane (containing 5% toluene), and 
      then the extract was purified with a propylsulfonic acid silica (PRS) column and 
      a C18 SPE column, and finally, the HAAs were stored in a methanol-ammonia 
      solution. The separation was achieved by using a TSK-gel ODS-80 column and a 
      gradient elution with the mobile phases of acetonitrile and 0.05 mol/L acetic 
      acid-ammonium acetate buffer (pH 3.5). The identification and quantitative 
      analysis of the HAAs fraction were carried out using an HPLC system with 
      ultraviolet-fluorescence detectors. The results showed that the correlation 
      coefficients of the 10 HAAs were all above 0.999 and the limits of detection were 
      in the range from 0.02 to 2.46 ng/g. The recoveries of the 10 HAAs spiked in beef 
      samples were 61.69% - 101.81% with the relative standard deviations (RSDs) 
      between 0.28% and 7.81%. 1-Methyl-9H-pyrido[4,3-b] indole (Harman) and 9H-pyrido 
      [4,3-b]indole (Norharman) were detected in all beef jerky, and the total HAAs 
      content of beef jerky were between 16.65 and 60.38 ng/g. This method is with wide 
      linear range and high sensitivity, and is enough for the analysis of the HAAs in 
      actual meat samples.
FAU - Wan, Kehui
AU  - Wan K
AD  - College of Food Science and Technology, Nanjing Agricultural University, Nanjing 
      210095, China.
FAU - Peng, Zengqi
AU  - Peng Z
FAU - Shao, Bin
AU  - Shao B
FAU - Yao, Yao
AU  - Yao Y
FAU - Shi, Jinming
AU  - Shi J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Amines)
RN  - 0 (Heterocyclic Compounds)
SB  - IM
MH  - Amines/*analysis/chemistry
MH  - Animals
MH  - Cattle
MH  - *Chromatography, High Pressure Liquid
MH  - Food Contamination/*analysis
MH  - Heterocyclic Compounds/*analysis/chemistry
MH  - Meat Products/*analysis
MH  - Solid Phase Extraction
EDAT- 2012/06/22 06:00
MHDA- 2014/07/31 06:00
CRDT- 2012/06/22 06:00
PHST- 2012/06/22 06:00 [entrez]
PHST- 2012/06/22 06:00 [pubmed]
PHST- 2014/07/31 06:00 [medline]
AID - 10.3724/sp.j.1123.2011.11039 [doi]
PST - ppublish
SO  - Se Pu. 2012 Mar;30(3):285-91. doi: 10.3724/sp.j.1123.2011.11039.