PMID- 22715696
OWN - NLM
STAT- MEDLINE
DCOM- 20140730
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 3
DP  - 2012 Mar
TI  - [Simultaneous determination of five synthetic sweeteners in food by solid phase 
      extraction-high performance liquid chromatography-evaporative light scattering 
      detection].
PG  - 292-7
AB  - A high performance liquid chromatographic method with evaporative light 
      scattering detection (HPLC-ELSD) was developed for the simultaneous determination 
      of five synthetic sweeteners (acesulfame-K, saccharin sodium, sodium cyclamate, 
      sucralose and aspartame) in food. The sweeteners were extracted by 0.1% (v/v) 
      formic acid buffer solution. The extract of sample was cleaned up and 
      concentrated with solid phase extraction (SPE) cartridge. Then the sweeteners 
      were separated on a C18 column (3 microm) using 0.1% (v/v) formic acid buffer 
      (adjusted to pH = 3.5 with aqueous ammonia solution)-methanol (61: 39, v/v) as 
      mobile phase, and finally detected by ELSD. The results showed that the 
      reasonable linearity was achieved for all the analytes over the range of 30 - 
      1000 mg/L with the correlation coefficients (r) greater than 0.997. The 
      recoveries for the five sweeteners ranged from 85.6% to 109.0% at three spiked 
      concentrations with the relative standard deviations (RSDs) lower than 4.0%. The 
      limits of detection (LODs, S/N = 3) were 2.5 mg/L for both acesulfame-K and 
      sucralose, 3 mg/L for saccharin sodium, 10 mg/L for sodium cyclamate, and 5 mg/L 
      for aspartame. The method is simple, sensitive and low cost, and has been 
      successfully applied to the simultaneous determination of the five synthetic 
      sweeteners in food.
FAU - Liu, Fang
AU  - Liu F
AD  - School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
FAU - Wang, Yan
AU  - Wang Y
FAU - Wang, Yuhong
AU  - Wang Y
FAU - Zhou, Junyi
AU  - Zhou J
FAU - Yan, Chao
AU  - Yan C
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Non-Nutritive Sweeteners)
SB  - IM
MH  - *Chromatography, High Pressure Liquid
MH  - *Food Safety
MH  - Light
MH  - Non-Nutritive Sweeteners/*analysis
MH  - *Scattering, Radiation
MH  - Solid Phase Extraction
EDAT- 2012/06/22 06:00
MHDA- 2014/07/31 06:00
CRDT- 2012/06/22 06:00
PHST- 2012/06/22 06:00 [entrez]
PHST- 2012/06/22 06:00 [pubmed]
PHST- 2014/07/31 06:00 [medline]
AID - 10.3724/sp.j.1123.2011.11034 [doi]
PST - ppublish
SO  - Se Pu. 2012 Mar;30(3):292-7. doi: 10.3724/sp.j.1123.2011.11034.