PMID- 22715992
OWN - NLM
STAT- MEDLINE
DCOM- 20121022
LR  - 20160511
IS  - 1945-0257 (Electronic)
IS  - 1945-0257 (Linking)
VI  - 16
IP  - 6
DP  - 2012 Jun
TI  - Association of interleukin-10 gene promoter polymorphism in allergic patients.
PG  - 632-5
LID - 10.1089/gtmb.2011.0255 [doi]
AB  - AIM: Allergic diseases are increasing alarmingly worldwide affecting >30% of the 
      population, including India. Allergy is the result of interaction of the epitopes 
      on the protein with the immunoglobulin E (IgE). T helper cell-2 cytokines promote 
      allergen-specific IgE antibody and induce eosinophil-dominated inflammatory 
      tissue responses. Interleukin-10 (IL-10), an antiinflammatory cytokine, plays a 
      major role in the development of the allergy. The cytokine gene polymorphism of 
      -592C-->A (rs1800872) and -1082G-->A (rs1800896) of IL-10 may influence the 
      expression of the protein. Hence, the current study was aimed to evaluate the 
      persistent association between these variants in the susceptibility of the 
      disease. METHODS: The allelic and genotype frequencies corresponding to IL-10 
      (-592C-->A; -1082G-->A) were determined in 94 allergic patients and 100 controls. 
      Genomic typing was performed with polymerase chain reaction with 
      sequence-specific primers. RESULT: The genotype AA at -592 position (p<0.000; 
      odds ratio [OR] 9.92; 95% confidence interval [CI]=5.06-19.42) and GG at 
      IL-10-1082 position (p<0.04; OR=2.47; 95% CI=1.003-4.96) was associated 
      significantly in patients compared with controls. A considerable frequency of A-A 
      haplotype in the patients and C-A, C-G haplotypes in controls was observed. A 
      highly noteworthy difference was found in diplotype frequencies of A/A-A/A and 
      A/A-G/A in patients and A/C-G/G and A/C-G/A in the controls. CONCLUSION: Our 
      results indicate that haplotype and diplotype frequencies of the IL-10 locus may 
      confer susceptibility to allergic patients.
FAU - Gaddam, Suman Latha
AU  - Gaddam SL
AD  - Bhagwan Mahavir Medical Research Centre, Hyderabad, India.
FAU - Priya, V Hari Sai
AU  - Priya VH
FAU - Babu, B M V Srikanth
AU  - Babu BM
FAU - Joshi, Lavanya
AU  - Joshi L
FAU - Venkatasubramanian, Sambasivan
AU  - Venkatasubramanian S
FAU - Valluri, Vijayalakshmi
AU  - Valluri V
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Genet Test Mol Biomarkers
JT  - Genetic testing and molecular biomarkers
JID - 101494210
RN  - 0 (DNA Primers)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Adult
MH  - DNA Primers/genetics
MH  - Female
MH  - Gene Frequency
MH  - *Genetic Predisposition to Disease
MH  - Genotype
MH  - Haplotypes
MH  - Humans
MH  - Hypersensitivity/*genetics
MH  - India
MH  - Interleukin-10/*genetics
MH  - Male
MH  - Polymerase Chain Reaction
MH  - *Polymorphism, Genetic
MH  - Promoter Regions, Genetic/*genetics
EDAT- 2012/06/22 06:00
MHDA- 2012/10/23 06:00
CRDT- 2012/06/22 06:00
PHST- 2012/06/22 06:00 [entrez]
PHST- 2012/06/22 06:00 [pubmed]
PHST- 2012/10/23 06:00 [medline]
AID - 10.1089/gtmb.2011.0255 [doi]
PST - ppublish
SO  - Genet Test Mol Biomarkers. 2012 Jun;16(6):632-5. doi: 10.1089/gtmb.2011.0255.