PMID- 22717633
OWN - NLM
STAT- MEDLINE
DCOM- 20150516
LR  - 20181201
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 64
IP  - 3
DP  - 2012 Jun 25
TI  - [Adiponectin up-regulates the expression of T-cadherin in cardiomyocytes injured 
      by hypoxia/reoxygenation].
PG  - 296-302
AB  - The aim of the present study was to investigate the effects of adiponectin (APN) 
      on the expression of T-cadherin in cultured Sprague-Dawley (SD) rat 
      cardiomyocytes injured by hypoxia/reoxygenation (H/R). Primary myocardial cells 
      from neonatal rats were obtained by enzymatic digestion. The cells were divided 
      into control group, H/R group and H/R+APN (3, 10, 20 and 30 mug/mL) groups. The 
      H/R group was incubated in anoxic environment (anoxic solution saturated with 
      high concentration N2) for 3 h, and then in the reoxygenation environment (the 
      reoxygenation solution saturated with pure oxygen) for 1 h. The H/R+APN group was 
      pretreated with different concentrations of APN for 24 h prior to the initiation 
      of H/R. The content of lactate dehydrogenase (LDH) was measured by chemistry 
      chromatometry. Cellular apoptosis was analyzed by flow cytometry and terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). The 
      expression of T-cadherin was detected by RT-PCR and Western blotting. The results 
      showed that, compared with control group, the apoptotic rate and release of LDH 
      were significantly increased in the H/R group, whereas the expressions of T-cad 
      mRNA and protein were decreased. Pretreating with APN significantly and 
      dose-dependently decreased apoptotic rate and LDH release, and up-regulated T-cad 
      mRNA and protein level in rat neonatal cardiomyocytes under H/R conditions. These 
      results suggest that APN may protect cardiomyocytes against H/R-induced injury by 
      up-regulating H/R-decreased T-cad expression.
FAU - Wang, Min
AU  - Wang M
AD  - Department of Cardiology, the Second Affiliated Hospital of Shanxi Medical 
      University, Taiyuan, China.
FAU - Chai, Ying-Ru
AU  - Chai YR
FAU - Xiao, Chuan-Shi
AU  - Xiao CS
FAU - Zhao, Xu-Jing
AU  - Zhao XJ
FAU - Wei, Na
AU  - Wei N
FAU - Bai, Rui
AU  - Bai R
FAU - Bian, Yun-Fei
AU  - Bian YF
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
RN  - 0 (Adiponectin)
RN  - 0 (Cadherins)
RN  - 0 (H-cadherin)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Adiponectin/*pharmacology
MH  - Animals
MH  - Apoptosis
MH  - Cadherins/*metabolism
MH  - Cell Hypoxia
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Myocytes, Cardiac/drug effects/*metabolism/pathology
MH  - Oxygen/adverse effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Up-Regulation
EDAT- 2012/06/22 06:00
MHDA- 2015/05/20 06:00
CRDT- 2012/06/22 06:00
PHST- 2012/06/22 06:00 [entrez]
PHST- 2012/06/22 06:00 [pubmed]
PHST- 2015/05/20 06:00 [medline]
PST - ppublish
SO  - Sheng Li Xue Bao. 2012 Jun 25;64(3):296-302.