PMID- 22718261
OWN - NLM
STAT- MEDLINE
DCOM- 20130611
LR  - 20230203
IS  - 1935-3456 (Electronic)
IS  - 1933-0219 (Print)
IS  - 1933-0219 (Linking)
VI  - 6
IP  - 1
DP  - 2013 Jan
TI  - Reduced severity of peanut-induced anaphylaxis in TLR9-deficient mice is 
      associated with selective defects in humoral immunity.
PG  - 114-21
LID - 10.1038/mi.2012.55 [doi]
AB  - Signaling through the innate immune system can promote or suppress allergic 
      sensitization. Toll-like receptor 9 (TLR9) has modulatory effects on the mucosal 
      immune system, and we hypothesized that TLR9 would influence susceptibility to 
      allergic sensitization to foods. We observed that TLR9-/- mice were resistant to 
      peanut-induced anaphylaxis. This was associated with a significant impairment in 
      total immunoglobulin E (IgE) and peanut-specific IgE and IgA, but not IgG1 or Th2 
      cytokine production. TLR9-/- mice had reduced development of Peyer's patches, but 
      resistance to sensitization was not restricted to oral routes. Rag1-deficient 
      mice were reconstituted with TLR9+/+ or -/- B cells plus CD4+ T cells. TLR9-/- B 
      cells regained the ability to produce IgE in the presence of a wild-type 
      environment. Our results demonstrate that TLR9 on an unknown cell type is 
      required for the development of IgE-producing B cells, and we conclude that TLR9 
      signaling indirectly shapes the immune response for optimal IgE production.
FAU - Berin, M C
AU  - Berin MC
AD  - Pediatric Allergy and Immunology, Immunology Institute, Mount Sinai School of 
      Medicine, New York, New York, USA. Cecilia.Berin@mssm.edu
FAU - Wang, W
AU  - Wang W
LA  - eng
GR  - U19 AI044236/AI/NIAID NIH HHS/United States
GR  - R01 AI093577/AI/NIAID NIH HHS/United States
GR  - U19 AI066738/AI/NIAID NIH HHS/United States
GR  - U19AI66738/AI/NIAID NIH HHS/United States
GR  - U19AI044236/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120620
PL  - United States
TA  - Mucosal Immunol
JT  - Mucosal immunology
JID - 101299742
RN  - 0 (Toll-Like Receptor 9)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Anaphylaxis/*genetics/*immunology/metabolism
MH  - Animals
MH  - B-Lymphocytes/immunology/metabolism
MH  - Disease Models, Animal
MH  - Disease Susceptibility
MH  - Gastrointestinal Tract/immunology/metabolism
MH  - Immunity, Humoral/*genetics
MH  - Immunoglobulin E/immunology/metabolism
MH  - Lymphocyte Activation/immunology
MH  - Mast Cells/immunology/metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Peanut Hypersensitivity/*genetics/*immunology/metabolism
MH  - Peyer's Patches/immunology/metabolism
MH  - Toll-Like Receptor 9/*deficiency/genetics/metabolism
PMC - PMC3449039
MID - NIHMS383258
EDAT- 2012/06/22 06:00
MHDA- 2013/06/12 06:00
PMCR- 2013/07/01
CRDT- 2012/06/22 06:00
PHST- 2012/06/22 06:00 [entrez]
PHST- 2012/06/22 06:00 [pubmed]
PHST- 2013/06/12 06:00 [medline]
PHST- 2013/07/01 00:00 [pmc-release]
AID - S1933-0219(22)01171-0 [pii]
AID - 10.1038/mi.2012.55 [doi]
PST - ppublish
SO  - Mucosal Immunol. 2013 Jan;6(1):114-21. doi: 10.1038/mi.2012.55. Epub 2012 Jun 20.