PMID- 22718280
OWN - NLM
STAT- MEDLINE
DCOM- 20130111
LR  - 20231213
IS  - 1460-2350 (Electronic)
IS  - 0268-1161 (Linking)
VI  - 27
IP  - 9
DP  - 2012 Sep
TI  - Trophoblast cells induce a tolerogenic profile in dendritic cells.
PG  - 2598-606
LID - 10.1093/humrep/des208 [doi]
AB  - BACKGROUND: Dendritic cells (DCs), which are biased toward a tolerogenic profile, 
      play a pivotal role in tissue-remodeling processes and angiogenesis at the 
      maternal-fetal interface. Here, we analyzed the effect of trophoblast cells on 
      the functional profile of DCs to gain insight on the tolerogenic mechanisms 
      underlying the human placental-maternal dialog at early stages of gestation. 
      METHODS: DCs were differentiated from peripheral blood monocytes obtained from 
      fertile women (n = 21), in the presence of interleukin (IL)-4 and 
      granulocyte-macrophage colony-stimulating factor during 5 days in culture. Then, 
      DCs were cultured with trophoblast cells (Swan-71 cell line obtained from normal 
      cytotrophoblast, at 7 weeks) for 24 h and for an additional 24 h in the absence 
      or presence of lipopolysaccharide (LPS) from Escherichia coli. DCs were recovered 
      and used for flow cytometry, enzyme-linked immunosorbent assay, RT-PCR and 
      suppression and migration assays. RESULTS: Trophoblast cells significantly 
      prevented the increase in CD83 expression induced by LPS without affecting the 
      expression of CD86, CD40 and human leukocyte antigen-DR (P < 0.05). Trophoblast 
      cells significantly decreased the production of IL-12p70 and tumor necrosis 
      factor-alpha, while it increased the production of IL-10 (P < 0.05). No changes were 
      observed in the production of IL-6 and monocyte chemotactic protein-1. The 
      culture of DCs with trophoblast cells, also suppressed the stimulation of the 
      allogeneic response triggered by LPS (P < 0.05). Conditioned DCs were able to 
      increase the frequency of CD4 + CD25 + Foxp3 cells and this effect was 
      accompanied by an increase in indoleamine 2, 3-dioxygenase expression in DCs (P < 
      0.05). CONCLUSIONS: The interaction of DCs with trophoblast cells promotes the 
      differentiation of DCs into cells with a predominantly tolerogenic profile that 
      could contribute to a tolerogenic microenvironment at the maternal-fetal 
      interface.
FAU - Salamone, Gabriela
AU  - Salamone G
AD  - Immunology Department, Instituto de Investigaciones Hematologicas and Instituto 
      de Estudios Oncologicos Fundacion Maissa, Academia Nacional de Medicina, Buenos 
      Aires, Argentina.
FAU - Fraccaroli, Laura
AU  - Fraccaroli L
FAU - Gori, Soledad
AU  - Gori S
FAU - Grasso, Esteban
AU  - Grasso E
FAU - Paparini, Daniel
AU  - Paparini D
FAU - Geffner, Jorge
AU  - Geffner J
FAU - Perez Leiros, Claudia
AU  - Perez Leiros C
FAU - Ramhorst, Rosanna
AU  - Ramhorst R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120620
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Antigens, CD)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD40 Antigens)
RN  - 0 (Immunoglobulins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Antigens, CD/biosynthesis
MH  - B7-2 Antigen/biosynthesis
MH  - CD40 Antigens/biosynthesis
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology
MH  - Female
MH  - Flow Cytometry/methods
MH  - Fluorescein-5-isothiocyanate
MH  - *Gene Expression Regulation
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - Humans
MH  - Immunoglobulins/biosynthesis
MH  - Interleukin-4/metabolism
MH  - Lipopolysaccharides/metabolism
MH  - Membrane Glycoproteins/biosynthesis
MH  - Models, Biological
MH  - Neovascularization, Pathologic
MH  - Trophoblasts/*metabolism
MH  - CD83 Antigen
EDAT- 2012/06/22 06:00
MHDA- 2013/01/12 06:00
CRDT- 2012/06/22 06:00
PHST- 2012/06/22 06:00 [entrez]
PHST- 2012/06/22 06:00 [pubmed]
PHST- 2013/01/12 06:00 [medline]
AID - des208 [pii]
AID - 10.1093/humrep/des208 [doi]
PST - ppublish
SO  - Hum Reprod. 2012 Sep;27(9):2598-606. doi: 10.1093/humrep/des208. Epub 2012 Jun 
      20.